Toxicity assessment of microcystin-leucine arginine in planarian Dugesia japonica  

作　　者：Baijie JIN Xiangjun LI Qingling ZHANG Wen ZHOU Yingyu LIU Zimei DONG Guangwen CHEN Dezeng LIU 

机构地区：[1]College of Life Science,Henan Normal University,Xinxiang,Henan,China

出　　处：《Integrative Zoology》2024年第6期1135-1150,共16页整合动物学(英文版)

基　　金：supported by the National Natural Science Foundation of China(Nos.32070427,32270501,32200376,and 31570376);the Major Public Welfare Project of Henan Province(201300311700);the Puyang Field Scientific Observation and Research Station for Yellow River Wetland Ecosystem,Henan Province.

摘　　要：Microcystin-leucine arginine(MC-LR),a representative cyanobacterial toxin,poses an increasing and serious threat to aquatic ecosystems.Despite investigating its toxic effects in various organisms and cells,the toxicity to tissue regeneration and stem cells in vivo still needs to be explored.Planarians are ideal regeneration and toxicology research models and have profound implications in ecotoxicology evaluation.This study conducted a systemic toxicity evaluation of MC-LR,including morphological changes,growth,regeneration,and the underlying cellular and molecular changes after MC-LR exposure,which were investigated in planarians.The results showed that exposure to MC-LR led to time-and dose-dependent lethal morphological changes,tissue damage,degrowth,and delayed regeneration in planarians.Furthermore,MC-LR exposure disturbed the activities of antioxidants,including total superoxide dismutase,catalase,glutathione peroxidase,glutathione S-transferase,and total antioxidant capacity,leading to oxidative stress and DNA damage,and then reduced the number of dividing neoblasts and promoted apoptosis.The results demonstrated that oxidative stress and DNA damage induced by MC-LR exposure caused apoptosis.Excessive apoptosis and suppressed neoblast activity led to severe homeostasis imbalance.This study explores the underlying mechanism of MC-LR toxicity in planarians and provides a basis for the toxicity assessment of MC-LR to aquatic organisms and ecological risk evaluation.

关 键 词：homeostasis MC-LR NEOBLAST oxidative stress PLANARIAN 

分 类 号：X52[环境科学与工程—环境工程]