Dual‐modality imaging for identifying thrombosis via platelet GPIIb/IIIa receptor targeted cyclic RGDfK  

作　　者：Yabin Wang Yang Zhang Ning Yang Xiaoting Zhang Sai Ma Mengqi Xu Yunxue Xu Shan Gao Yan Fang Na Li Sulei Li Ping Liang Xu Zhang Li Fan Feng Cao 

机构地区：[1]Department of Cardiology&National Clinical Research Center for Geriatric Diseases,2nd Medical Center of Chinese PLA General Hospital,Beijing,China [2]Chinese PLA Medical College&Department of Cardiology,National Clinical Research Center for Geriatric Diseases,Beijing,China [3]Department of Biomedical Engineering,College of Engineering,Peking University,Beijing,China [4]Department of Cardiology,Jinling Hospital,Nanjing,Jiangsu,China [5]Department of Interventional Ultrasound,Chinese PLA General Hospital,Beijing,China [6]Senior Department of Urology,The Third Medical Center of Chinese PLA General Hospital,Beijing,China

出　　处：《iRADIOLOGY》2024年第3期227-239,共13页融合影像学(英文)

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:82270533,U22A6008,81970443;National Key Research and Development Program of China,Grant/Award Number:2022YFC3602400;Key Health Care Project of National Health,Grant/Award Number:2020ZD05;Basic Research Reinforcement Project,Grant/Award Number:JCJQ‐JJ‐1079。

摘　　要：Background:Acute thrombotic events play a major role in various cardio-vascular diseases.Therefore,direct thrombus imaging can be proved beneficial for early diagnosis and prompt therapy of thrombosis.Our study investigated targeted dual‐modality cyclic arginine‐glycine‐aspartic micro bubbles(cRGD‐MBs)for direct imaging of thrombi by fluorescence and ultrasound.Methods:cRGD‐MBs were prepared by mechanical vibration and chemical chelation methods.Results:Coulter counter analysis demonstrated that the cRGD‐MBs were well dispersed,with diameters ranging from 1 to 3μm.They emitted bright red fluorescence under an excitation wavelength of 660 nm.In vivo fluorescence and ultrasound imaging revealed that cRGD‐MBs accumulated at the site of thrombus in the carotid artery with significant fluorescence and ultrasonic signal.Conclusions:This study showed that novel microbubble cRGD‐MBs were successfully synthesized,and that these could potentially be used as contrast agents for immediate diagnosis of acute thrombus in vivo.

关 键 词：GPIIb/IIIa receptor MICROBUBBLE molecular imaging PLATELET THROMBOSIS 

分 类 号：TQ3[化学工程]