基于Lambert-Beer定律的吸光度监测微容量液体体积测量系统及方法研究  

Research on Absorbance Monitoring Micro Liquid Volume Measurement System and Method Based on Lambert-Beer Law

作　　者：常旭 王金涛[1] 刘翔[1] 张竟月 佟林[1] 郭立功[1] 许常红[1] 时文才[1] 徐浩铭 CHANG Xu;WANG Jintao;LIU Xiang;ZHANG Jingyue;TONG Lin;GUO Ligong;XU Changhong;SHI Wencai;XU Haoming(National Institute of Metrology,Beijing 100029,China)

机构地区：[1]中国计量科学研究院,北京100029

出　　处：《计量学报》2025年第2期198-203,共6页Acta Metrologica Sinica

基　　金：国家重点研发计划(2023YFF0615500);国家自然科学基金(52075514)。

摘　　要：为解决静力称量法对环境要求高、液体蒸发影响精度且难以实现现场检测的问题,基于Lambert-Beer定律研究了微容量液体体积测量方法。为了提高该方法的测量重复性,设计了具有吸光度监测光路的测量系统,用于监测光源波动等干扰因素并提出了修正系数。设计了测量方案,并给出了相应数学解析模型,以实现微容量液体体积的计算。搭建了实际测量系统并进行实验,液体混匀实验验证了所设计的测量系统可有效提高测量效率,针对100μL微容量液体体积检定点开展的测量实验,验证了吸光度监测光路可以把重复性从2.02%提高至0.76%,最终测量结果符合JJG 646—2006的要求,验证了测量系统的有效性。To overcome the shortcoming of hydro-weighing method,which has high environmental requirements,liquid evaporation affects accuracy and is difficult to realize field detection,a micro liquid volume measurement method was studied based on Lambert-Beer.To improve measurement repeatability,a measurement system with absorbance monitoring optical path was designed to monitor interference factors such as light source fluctuations,and a correction coefficient was proposed.A measurement scheme was designed,and corresponding mathematical analytical model was proposed to calculate micro liquid volume.A practical measurement system was built and experiments were carried out.The liquid mixing experiment verified that the measurement efficiency was improved.The measurement experiments carried out on the volume detection point of 100μL verified that the absorbance monitoring optical path can improve the repeatability from 2.02%to 0.76%.The final measurement results meet the requirements of JJG 646-2006,which verifies the effectiveness of the measurement system.

关 键 词：容量计量 微容量液体体积 Lambert-Beer定律 吸光度监测 

分 类 号：TB938.3[一般工业技术—计量学]