富硒石斛内生菌筛选及其富硒动态变化  

Screening of Selenium-enriched Dendrobium Endophytes and Its Dynamic Changes of Selenium Enrichment

作　　者：刘如振 徐炜 骆祝华 郭迟鸣 王杰[1] 林文珍[1] LIU Ru-zhen;XU Wei;LUO Zhu-hua;GUO Chi-ming;WANG Jie;LIN Wen-zhen(Fujian Institute of Subtropical Botany/Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant,Xiamen 361006,Fujian China;Key Laboratory of Marine Biogenetic Resource,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,Fujian China)

机构地区：[1]福建省亚热带植物研究所/福建省亚热带植物生理生化重点实验室,福建厦门361006 [2]自然资源部第三海洋研究所海洋生物遗传资源重点实验室,福建厦门361005

出　　处：《亚热带植物科学》2024年第6期537-544,共8页Subtropical Plant Science

基　　金：厦门市科技计划项目(3502Z20204503-9、3502Z20182011);基本科研项目(市级)(3502Z2024YZS03、3502Z2024YZS07);福建省林业科技项目(闽林科便函[2022]9号)。

摘　　要：从铁皮石斛Dendrobium officinale中筛选对硒具有高耐受性和富集能力的菌株,并对其进行菌种鉴定及硒富集动态变化研究,为富硒微生物种质资源改良和提高植物硒含量提供理论依据。首先,通过16S rDNA序列分析鉴定菌株BK-1的种类,随后评估该菌株对亚硒酸钠耐受力,测试其耐受浓度,分析菌株在硒富集过程中将无机硒转化为有机硒的能力,测定其总硒含量、有机硒产量和转化率,最后优化添加硒离子浓度。菌株BK-1被鉴定为唐菖蒲伯克霍尔德氏菌Burkholderia gladioli。该菌株对亚硒酸钠的耐受浓度高达130μg·m L^(-1)。在富硒过程中,BK-1菌株在48 h内总硒含量达577.74μg·g^(-1)DCW,有机硒产量为505.66μg·g^(-1)DCW,转化率87.42%。添加硒离子显著缩短菌株的对数生长期并减少菌体生长量,最适硒离子浓度为50μg·mL^(-1)。This study aimed to identify and characterize strains from Dendrobium officinale with high selenium tolerance and enrichment capacity,and to investigate the dynamics of selenium accumulation in these strains.The findings are intended to provide a theoretical foundation for the improvement of selenium-enriched microbial germplasm and for enhancing selenium content in plants.The strain BK-1 was initially identified through 16S rDNA sequence analysis.The strain’s tolerance to sodium selenite was then evaluated,determining its maximum tolerance concentration.The ability of the strain to convert inorganic selenium into organic selenium during the enrichment process was assessed,along with measurements of total selenium content,organic selenium yield,and conversion efficiency.An optimization experiment was also conducted to establish the optimal selenium ion concentration for supplementation.Strain BK-1 was identified as Burkholderia gladioli.This strain demonstrated a high tolerance to sodium selenite,with a maximum tolerance concentration of 130μg·mL^(-1).During the selenium enrichment process,strain BK-1 achieved a total selenium content of 577.74μg·g-1 DCW(dry cell weight)within 48 hours,with an organic selenium yield of 505.66μg·g-1 DCW and a conversion efficiency of 87.42%.The addition of selenium ions significantly shortened the logarithmic growth phase and reduced biomass,with the optimal selenium ion concentration determined to be 50μg·mL^(-1).

关 键 词：内生菌 富硒 唐菖蒲伯克霍尔德氏菌 硒产量 

分 类 号：R931[医药卫生—生药学]