匙羹藤多糖超声辅助低共熔溶剂提取工艺优化及其活性测定  

Ultrasound-Assisted Deep Eutectic Solvent Extraction of Gymnema sylvestre Polysaccharides and Its Activity Determination

作　　者：郝胜杰 后海庆 尹杰 李洪森 杜雨欣 靳枫阳 李欠 陈桂林[2] HAO Sheng-jie;HOU Hai-qing;YIN Jie;LI Hong-sen;DU Yu-xin;JIN Feng-yang;LI Qian;CHEN Gui-lin(College of Agriculture/State Key Laboratory of Crop Stress Biology for Arid Areas,Gansu Agricultural University,Lanzhou 730070,Gansu China;Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,Hubei China)

机构地区：[1]甘肃农业大学农学院/干旱生境作物学国家重点实验室,甘肃兰州730070 [2]中国科学院武汉植物园,湖北武汉430074

出　　处：《亚热带植物科学》2024年第6期545-553,共9页Subtropical Plant Science

基　　金：湖北省自然科学基金(2022CFB450);中国科学院大学生科创训练项目(No.20234002921);国家级大学生创新创业训练计划&甘肃省重点支持领域项目(202410733002)。

摘　　要：优化超声辅助低共熔溶剂(DESs)提取匙羹藤多糖工艺,探究其生物活性,为匙羹藤产品开发提供理论依据。以匙羹藤多糖提取率为指标,在单因素实验基础上,采用Box-Behnken响应面法对提取工艺进行优化,并研究匙羹藤多糖对DPPH、ABTS^(+)、FRAR和H_(2)O_(2)自由基的清除能力,对α-淀粉酶和α-葡萄糖苷酶活性的抑制率,以及对几种细菌的抗菌作用。结果显示,氯化胆碱和柠檬酸合成的DESs提取率最高,经响应面法优化后,在氯化胆碱与柠檬酸物质的量比1:1.02、含水量39.32%、液料比20.21:1、超声时间39.23 min、超声温度41.35℃、超声功率412 W条件下,匙羹藤多糖提取率达29.23%,比传统水提法提升近15倍。体外活性实验表明匙羹藤多糖具有较高的抗氧化活性、广谱抗菌能力及酶抑制活性,对未来开发新型降糖产品具有较高潜力。DESs具有绿色环保、高效且重复利用等优点,作为天然产物的提取介质,能有效提升匙羹藤多糖提取效率。Optimization of ultrasonic-assisted deep eutectic solvents(DESs)extraction process for Gymnema sylvestre polysaccharides was conducted to investigate its biological activity,to provide theoretical support for the development of G.sylvestre products.Using the polysaccharide extraction yield as the evaluation index,the Box-Behnken response surface method was applied to optimize the extraction process based on single-factor experiments.Additionally,the antioxidant activities of the polysaccharides were evaluated by their ability to scavenge DPPH,ABTS^(+),FRAP,and H_(2)O_(2) radicals,while the inhibitory effects onα-amylase andα-glucosidase,as well as antibacterial activity against several bacteria strains,were also studied.The results showed that the DESs synthesized from choline chloride and citric acid achieved the highest extraction rate.After optimization using the response surface method,under conditions where the molar ratio of choline chloride to citric acid was 1:1.02,water content was 39.32%,liquid-to-material ratio was 20.21:1,ultrasonic time was 39.23 min,ultrasonic temperature was 41.35℃,and ultrasonic power was 412 W,the extraction yield of G.sylvestre polysaccharides reached 29.23%,which was nearly 15 times higher than the traditional water extraction method.In vitro activity experiments demonstrated that G.sylvestre polysaccharides exhibited high antioxidant activity,broad-spectrum antibacterial capacity,and enzyme inhibition activity,showing great potential for developing novel anti-diabetic products.DESs,being environmentally friendly,efficient,and reusable as extraction media for natural products,significantly improved the extraction efficiency of G.sylvestre polysaccharides.

关 键 词：匙羹藤 多糖 超声提取 低共熔溶剂 活性测定 

分 类 号：Q946.3[生物学—植物学]