银杏叶提取物对小鼠脑缺血-再灌注损伤的保护作用及机制研究  

Protective effect and mechanism of Ginkgo biloba extracts against cerebral ischemia-reperfusion injury

作　　者：汪雅玲 刘海林 文君 陈辉 王鹏 纪金金 WANG Ya-ling;LIU Hai-lin;WEN Jun;CHEN Hui;WANG Peng;JI Jin-jin(College of Adult Education,Chongqing Medical Science and Technology University,Chongqing 400061,China;Department of Pharmacy,People's Hospital of Chongqing Liangjiang New Area,Chongqing 401121,China;Kangmei Community Health Service Center of Chongqing Liangjiang New Area,Chongqing 401123,China)

机构地区：[1]重庆市医药科技学校成人教育学院,重庆400061 [2]重庆两江新区人民医院药学部,重庆401121 [3]重庆两江新区康美社区卫生服务中心,重庆401123

出　　处：《局解手术学杂志》2025年第3期212-218,共7页Journal of Regional Anatomy and Operative Surgery

基　　金：重庆市科技局自然科学基金(CSTB2024NSCQ-MSX 0085)。

摘　　要：目的运用网络药理学及动物实验探索银杏叶提取物(GB)治疗脑缺血再灌注(CIR)损伤的机制。方法运用TCMSP、GeneCards等数据库获取GB与CIR的交集靶点,利用Cytoscape软件、Metascape数据库进行相关靶点的分析及绘图。线栓法构建小鼠短暂性大脑中动脉栓塞(t-MCAO)模型,通过神经功能缺损评分、Morris水迷宫实验观察GB对t-MCAO后小鼠神经功能的影响。HE染色观察小鼠海马区域神经元病理结构变化,Western blot对网络药理学筛选的信号通路进行验证。结果网络药理学预测GB包含33个活性成分,其治疗CIR的潜在作用靶点包含Caspase3、Bax等116个靶点。此外,GB可能通过PI3K-Akt、AMPK等信号通路发挥其保护作用。动物实验表明,GB治疗能显著改善小鼠神经功能及学习空间记忆能力,减轻脑组织病理学损伤,并激活p-Akt/Akt信号通路。结论GB凭借其多靶点及多通路的特性治疗CIR,其可能通过激活p-Akt/Akt信号通路减少神经元凋亡。Objective To investigate the mechanism of Ginkgo biloba extracts(GB)in the treatment of cerebral ischemia-reperfusion(CIR)injury based on network pharmacology and animal experiments.Methods The intersection targets of CIR and GB were obtained from TCMSP,GeneCards and other databases.Cytoscape software and Metascape database were used to analyze and map the related targets.The model of transient middle cerebral arterial occlusion(t-MCAO)was constructed in mice by suture method,and the effects of GB on the neurological function of mice after t-MCAO were observed by the neurological deficit score and Morris water maze test.HE staining was used to observe the pathological structural changes of neuron in the hippocampus of mice,and Western blot was used to verify the signal pathways screened by network pharmacology.Results Network pharmacology predicted that GB contained 33 active ingredients,and 116 potential targets of GB in treatment of CIR included Caspase3,Bax,etc.In addition,GB may play a protective role through signaling pathways such as PI3K-Akt and AMPK.Animal experiments showed that GB treatment could significantly improve the neural function and learning spatial memory ability of mice,alleviate the brain histopathological injury,and activate p-Akt/Akt signaling pathway.Conclusion GB has the characteristics of multi-target and multi-pathways therapy for CIR,which may reduce neuronal apoptosis by activating the p-Akt/Akt signaling pathway.

关 键 词：脑缺血-再灌注损伤 银杏叶提取物 神经功能缺损 AKT 网络药理学 

分 类 号：R961.1[医药卫生—药理学]