茯苓多糖对上海白猪精液冷冻保存及精子DNA甲基化的影响  

Effects of Poria cocos polysaccharides on cryopreservation of Shanghai White Pig semen and DNA methylation of sperm

作　　者：孙玲伟 朱永军 周金勇 刘富琴 张德福[1,2] 戴建军 滑志民[3] SUN Lingwei;ZHU Yongjun;ZHOU Jinyong;LIU Fuqin;ZHANG Defu;DAI Jianjun;HUA Zhimin(Institute of Animal Husbandry and Veterinary Science,Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Agricultural Genetics and Breeding;Key Laboratory of Livestock and Poultry Resources(Pig)Evaluation and Utilization,Ministry of Agriculture and Rural Affairs,Shanghai 201106,China;Department of Animal Science and Technology,Shanghai Vocational College of Agriculture and Forestry,Shanghai 201699,China)

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海市农业遗传育种重点实验室,上海201106 [2]农业农村部畜禽资源(猪)评价利用重点实验室,上海201106 [3]上海农林职业技术学院动物科学技术系,上海201699

出　　处：《上海农业学报》2025年第1期72-79,共8页Acta Agriculturae Shanghai

基　　金：“十四五”国家重点研发计划(2021YFD120301);上海农林职业技术学院中青年领军人才计划(A6-02RS-23-34);重庆市技术创新与应用发展专项(CSTC2021JSCX-DXWTBX0004)。

摘　　要：为研究茯苓多糖(Poria cocos mushroom polysaccharides,PCP)在上海白猪精液冷冻保存中的作用,采集8头上海白猪24份精液,在精液冷冻液中添加不同浓度的PCP,测定冷冻-解冻后精液质量和抗氧化活性,以及冷冻保存后精子DNA甲基化水平。结果表明:与未添加PCP组相比,600μg∕mL PCP添加组精子活力最高(P<0.05),600μg∕mL PCP添加组精子活率最高(P<0.05),而600μg∕mL和900μg∕mL PCP添加组的精子顶体完整率、质膜完整率、线粒体活性、活性氧(ROS)和丙二醛(MDA)含量较高。此外,600μg∕mL PCP添加组精液中的超氧化歧化酶(SOD)活性最高(P<0.05),300、600、900μg∕mL PCP添加组精液中的过氧化氢酶(CAT)活性较高(P<0.05),600μg∕mL PCP添加组精液中的谷胱甘肽过氧化物酶(GSH-Px)活性最高(P<0.05);所有添加组的精子DNA甲基化水平均显著低于对照组(P<0.05)。综上,添加适量的PCP可以改善上海白猪的精液冷冻保存效果,也能够降低冷冻后精子的DNA甲基化水平。To study the effects ofPoria cocospolysaccharides(PCP)on cryopreservation of Shanghai White Pig semen and DNA methylation of sperm,24 ejaculates were collected by artificial vagina from 8 Shanghai White Pigs.Semen samples were diluted with tris-based extender supplemented with different concentrations of PCP(0,300,600,900,1200,1500μg∕mL).After thawing,sperm samples were assessed for semen quality,antioxidant activities and DNA methylation of sperm.The results showed that the addition of 600μg∕mL PCP into the extender led to a higher semen motility in comparison to the control(P<0.05).The addition of 600μg∕mL PCP into the extender led to a higher percentages of plasma membrane and acrosome integrities,and mitochondrial activity of sperm(P<0.05).Compared with the controls,the addition of 600μg∕mL and 900μg∕mL PCP significantly decreased the expressions of reactive oxygen species(ROS)and malondialdehyde(MDA)in sperm(P<0.05),the addition of 600μg∕mL PCP significantly increased the super oxidase dismutase(SOD)level in sperm(P<0.05),the addition of 300,600μg∕mL and 900μg∕mL PCP significantly increased the catalase(CAT)activity in sperm(P<0.05),and the addition of 600μg∕mL PCP significantly increased the activity of glutathione peroxidase(GSH-Px)in sperm(P<0.05).A significant decrease in the sperm DNA methylation levels was observed in all groups with the addition of PCP compared to the control(P<0.05).In summary,the addition of PCP to the extenders enhanced the post-thawed semen quality of Shanghai White Pig sperm and also improved the DNA methylation of sperm.

关 键 词：上海白猪 精液冷冻保存 茯苓多糖(PCP) 抗氧化 DNA甲基化 

分 类 号：S828[农业科学—畜牧学]