黄柏调节机体代谢的性效表征和机制研究  

Property-effect Relationship and Mechanism of Phellodendri Chinensis Cortex in Regulating Metabolism

作　　者：臧梦楠 孟晶[2] 谢冉 宋琦 刘星 宋海津 张焕焕 高旅 窦宝凯 张淼[1] 霍海如[2] 隋峰[2] ZANG Mengnan;MENG Jing;XIE Ran;SONG Qi;LIU Xing;SONG Haijin;ZHANG Huanhuan;GAO Lv;DOU Baokai;ZHANG Miao;HUO Hairu;SUI Feng(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国现代中药》2025年第3期546-553,共8页Modern Chinese Medicine

基　　金：国家自然科学基金面上项目(82173998,82374057);中国中医科学院科技创新工程项目(CI2021A03803)。

摘　　要：目的:探讨苦寒中药黄柏对机体代谢水平的影响,并揭示其分子作用机制。方法:采用昆明种小鼠灌胃适量黄柏水煎液(黄柏组)或0.9%氯化钠溶液(对照组),分别观测动物整体状态、核心温度及寒热趋向性的变化;基于超高效液相色谱-质谱法(UPLC-MS),检测不同组别小鼠血清中内源性代谢产物水平,比较血清的代谢图谱,并寻找差异代谢物;采用Cytoscape和Metscape软件对差异代谢物进行网络模块化分析,寻找可能的分子作用靶标。结果:性效表征监测中,黄柏组小鼠肛温明显下降,给药第1天与对照组相比差异有统计学意义(P<0.05);同时给药组小鼠停留在高温区的比例均高于对照组,其中第1天与对照组相比差异有统计学意义(P<0.01);分子作用机制研究中共发现19个差异代谢物,其中差异代谢物节点度值大于均值的有花生四烯酸、亚油酸和棕榈酸,通过相关代谢通路及网络模块化分析,花生四烯酸代谢通路、亚油酸代谢通路在代谢组学网络模块图中占比最大。结论:通过调节花生四烯酸、亚油酸、棕榈酸、异柠檬酸等分子靶点和关联生物网络进而下调机体代谢的整体代谢状态可能是黄柏表征苦寒药性的分子机制之一。Objective:To investigate the effect of the bitter cold Chinese herbal medicine Phellodendri Chinensis Cortex on body metabolism and reveal its molecular mechanism.Methods:Kunming mice were administrated with an appropriate amount of Phellodendri Chinensis Cortex decoction or normal saline(control group)by gavage,and the changes in the overall state,core temperature,and cold-heat tendency of the mice were observed.Ultra-performance liquid chromatography-mass spectrometry(UPLC-MS)was employed to measure the levels of endogenous metabolites in the serum samples of different groups of mice.The serum profiles were compared for identification of differential metabolites between the two groups.Cytoscape and Metscape were used for network modular analysis of differential metabolites to find potential molecular targets.Results:In the study of the property-effect relationship,the rectal temperatures of the mice in the Phellodendri Chinensis Cortex group declined compared with those in the control group,with significant differences on day 1(P<0.05).The proportion of staying in the high temperature area in the Phellodendri Chinensis Cortex group was higher than that in the control group,and the difference was significant on day 1(P<0.01).A total of 19 differential metabolites were identified,among which arachidonic acid,linoleic acid,and palmitic acid had node degrees greater than the mean value.The arachidonic acid and linoleic acid metabolic pathways accounted for the largest proportion in the module diagram of the metabolomic network.Conclusion:The bitter cold herbal medicine Phellodendri Chinensis Cortex may regulate molecular targets such as arachidonic acid,linoleic acid,palmitic acid,and isocitric acid and associated biological networks to down-regulate the overall state of body metabolism.

关 键 词：黄柏 机体代谢 温度趋向 内源性产物 代谢通路 

分 类 号：R285.5[医药卫生—中药学]