Livestock grazing is associated with the gut microbiota and antibiotic resistance genes in sympatric plateau pika(Ochotona curzoniae)  

作　　者：Jidong ZHAO Tuo FENG Xiaolei AN Xiaoning CHEN Ning HAN Jing WANG Gang CHANG Xiang HOU 

机构地区：[1]Shaanxi Key Laboratory of Qinling Ecological Security,Shaanxi Institute of Zoology,Xi’an,P.R.China

出　　处：《Integrative Zoology》2024年第4期646-661,共16页整合动物学(英文版)

基　　金：supported by the Second Tibetan Plateau Scientific Expedition and Research Program(STEP,2019QZKK0501);the National Natural Science Foundation of China(32172436);the Science and Technology Program of Shaanxi Academy of Science(2022K-16).

摘　　要：With the overuse of antibiotics in health care and animal husbandry,antibiotic resistance becomes a serious threat to public health.Antibiotic residues from veterinarymedicine have increased the dissemination of antibiotic resistance genes(ARGs)by horizontal gene transfer globally,leading to the enrichment of ARGs in wildlife.Plateau pika(Ochotona curzoniae)is a small herbivore endemic to the Qinghai–Tibetan Plateau.Previous studies reveal that pika evolves a coprophagy behavior toward cohabitated yak,which makes the pika population a potential reservoir of ARGs.Yet,little is known about the resistome of pika under different grazing intensities.Here,we sampled the cecum content of pika from three different grazing intensity areas in the Qinghai–Tibetan Plateau to evaluate the effect of grazing on its gut microbiota and resistome.By using the 16S full-length amplicon and metagenomic sequencing,our study revealed that livestock grazing significantly altered the gut microbial community of plateau pika as compared to prohibited grazing areas.We found bacterial lineage Prevotellaceae,Lachnospirales,and RF39 increased in grazing areas.Analysis of the resistome revealed that pika from continuous grazing areas enriched a higher abundance of colistin(MCR)and streptogramin(vat)resistance genes.Moreover,we observed significant correlations between the gut microbial community,ARGs,and mobile genetic element profiles,hinting that pika gut microbiota was an important shaping force of the resistome.In future studies,the continuous monitoring of wildlife gut resistome and environmental antibiotic residues is imperative for a better understanding and for tackling the horizontal gene transfer of ARGs across the wildlife–livestock interface.

关 键 词：antibiotic resistance genes livestock grazing mobile genetic elements plateau pika 

分 类 号：S81[农业科学—畜牧学]