油菜未成熟种子含水量近红外快速测定方法  

Rapid Near-infrared Determination of Water Content in Immature Seeds of Oilseed Rape

作　　者：赵培栋 舒添 刘沛 张振乾[1] 杨柳[1] ZHAO Peidong;SHU Tian;LIU Pei;ZHANG Zhenqian;YANG Liu(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128

出　　处：《作物研究》2025年第1期81-87,共7页Crop Research

基　　金：湖南省种业创新项目(2021NK1004)。

摘　　要：成熟期的含水量是判断油菜成熟度的重要指标,准确测定油菜种子的含水量是顺利开展油菜机械化收获的关键。为了建立油菜未成熟种子含水量测定的近红外光谱模型,本研究对2053个甘蓝型油菜终花后35~38、38~41 d的角果皮和种子脱水速率进行线性分析,利用烘干法测定终花后35、38、41 d的种子含水量,从中选取1423份终花后不同时期的甘蓝型油菜角果为材料,采用近红外光谱分析仪WINISI软件系统对数据进行分析和处理。结果表明:终花后35~38 d的油菜角果皮与种子脱水速率相关性显著,R^(2)=0.5682,终花后38~41 d的油菜角果皮与种子脱水速率相关性显著,R^(2)=0.6304。油菜成熟后期角果皮的含水量变化与种子的含水量变化密切相关,故以油菜种子材料建立近红外含水量无损检测模型,其校正标准差(SEC)和校正内部检验标准差(SECV)分别为2.7431和2.8740,校正决定系数(RSQ)和校正内部检验决定系数(1-VR)分别为0.9101和0.9012,均大于0.9,模型的回归曲线与实测值拟合度较高,具有显著相关性;外部检验相关系数R^(2)=0.8454,表明模型的预测值与真实值拟合度高,预测性好,可用于油菜未成熟种子含水量的测定。综合分析,本试验建立的近红外模型可准确、快速、无损地检测油菜未成熟种子含水量。The water content of rapeseed during maturity is an important indicator to judge the maturity of rapeseed.Accu-rately measuring the water content of rapeseed is the key to smooth mechanized rapeseed harvesting.In order to establish a near infrared spectroscopy model for measuring the water content of immature rapeseed seeds,A linear analysis of the dehy-dration rate of 2053 rapeseed pods and seeds at 35-38 and 38-41 days after final flowering was conducted in this stu-dy.The moisture content of rapeseed seeds at 35,38 and 41 days after final flowering was determined using seed drying method.Additionally,1423 samples of rapeseed pods at different stages after final flowering were selected as materials.The results showed that there was a significant correlation between rapeseed pod peel and seed dehydration rate at 35-38 days after final flowering,R^(2)=0.5682.There was a significant correlation between rapeseed pod peel and seed dehydration rate at 38-41 days after final flowering,R^(2)=0.6304.The correlation between pod peel and seed dehydration rate at 38-41 days after final flowering was more significant than that at 35-38 days after final flowering.The change of water content in the cornicle of rapeseed in late maturity is closely related to the change of water content in the grain.Therefore,a nea-rinfrared non-destructive testing model for water content was established based on rapeseed seed materials.The corrected standard deviation(SEC)and corrected internal test standard deviation(SECV)were 2.7431 and 2.8740 respectively,and the corrected determination coefficient(RSQ)and corrected internal test determination coefficient(1-VR)were 0.9101 and 0.9012 respectively,both greater than 0.9.The regression curve of the model has a high degree of fitting with the measured values and has a significant correlation;the external test correlation coefficient R^(2)=0.8454,indicating that the predicted values of the model have a high degree of fitting with the true values and good predictability,and can be used to determin

关 键 词：油菜 种子 含水量 快速检测 

分 类 号：S565.032[农业科学—作物学]