十滴水中非法添加色素的液相色谱-串联质谱检查法  

Inspection of Illegal Pigment Addition in Shidishui by LC-MS/MS

作　　者：孙彦敏 吴嫣艳 付裕 张妤琳 庞庆林 SUN Yanmin;WU Yanyan;FU Yu;ZHANG Yulin;PANG Qinglin(Jiangsu Institute for Food and Drug Control,Nanjing 210019,China)

机构地区：[1]江苏省食品药品监督检验研究院,南京210019

出　　处：《药学与临床研究》2025年第1期45-48,共4页Pharmaceutical and Clinical Research

基　　金：江苏省药品监督管理局科研计划项目(202304)。

摘　　要：目的:建立十滴水非法添加11种脂溶性和6种水溶性色素的液相色谱-串联质谱检测方法。方法:采用液相色谱仪-三重四级杆质谱法,Agilent Poroshell 120 EC-C_(18)(3.0 mm×100 mm,2.7μm)色谱柱,乙腈-0.1%甲酸水流动相梯度洗脱分离,流速为0.4 mL·min^(-1),柱温为40℃,进样体积为1μL;电喷雾正离子化多反应检测模式进行17种色素的含量测定。结果与结论:本检查法检出17种色素在各自范围内线性关系均良好(r>0.999),回收率为88.3%~111.5%,定量限为0.033 3~33.2 ng·mL^(-1)。在105批样品中,17批检出808猩红,10批检出罗丹明B。该方法灵敏度高,准确性好,适用于十滴水中非法添加色素检查。Objective:To establish an LC-MS/MS detection method for illegal addition of 11 kinds of lipid soluble pingments and 6 kinds of water soluble pigments in Shidishui.Methods:Liquid chromatogra-phy triple quadrupole mass spectrometry was performed with an Agilent Poroshell 120 EC-C_(18)(3.0 mm×100 mm,2.7μm)chromatographic column at 40℃.Acetonitrile-0.1%formic acid was applied for gradient elution separation with a flow rate at 0.4 mL·min^(-1).The injection volume was 1μL.The contents of 17 kinds of pigments were determined by electrospray positive ionization multi reaction detection mode.Results&Conclusion:The 17 kinds of pigments showed good linearities in selected concentration ranges(r>0.999).The recoveries were between 88.3%-111.5%,and the detection limits were respectively at 0.0333-33.2 ng·mL^(-1).Out of 105 batches of samples,Scarlet 808 and Rhodamine B were detected in 17 batches and 10 batches,respectively.The established inspection method for illegal addition of 17 types of pigments in Shidishui has simple pretreatment operation,high sensitivity and good accuracy,and is suitable for the inspection of illegal pigment addition in Shidishui.

关 键 词：十滴水 色素 非法添加 含量测定 液相色谱-串联质谱法 

分 类 号：R917[医药卫生—药物分析学]