基于HPLC-DAD的连翘败毒丸中7种成分含量测定及质量评价  

Content Determination and Quality Evaluation of Seven Components in Lianqiao Baidu Pills Based on HPLC-DAD

作　　者：张丽琴 崔庆德 吕晋 ZHANG Liqin;CUI Qingde;LV Jin(Qujing Certification Institute for Detection and Testing,Municipal Center for Drug,Medical Apparatus and Instruments Control,Qujing,Yunnan 655000,China)

机构地区：[1]曲靖市检验检测认证院药品和医疗器械检验检测中心,云南曲靖655000

出　　处：《药学与临床研究》2025年第1期58-61,共4页Pharmaceutical and Clinical Research

摘　　要：目的:建立高效液相色谱-二极管阵列检测器法(HPLC-DAD),同时测定连翘败毒丸中绿原酸、连翘酯苷A、秦皮乙素、栀子苷、黄芩苷、汉黄芩苷及芍药苷的含量,并比较不同厂家连翘败毒丸的质量差异。方法:供试品以甲醇超声提取40 min,采用依利特SinoChromODS-BPC18色谱柱(250 mm×4.6 mm,5μm),以0.1%磷酸-乙腈为流动相,在30℃的柱温下以1.0 mL·min^(-1)的流速梯度洗脱;进样量为15μL;波长切换法依次检测326 nm(绿原酸)、229 nm(秦皮乙素、栀子苷和芍药苷)、330 nm(连翘酯苷A和黄芩苷)和275 nm(汉黄芩苷)。结果:7种成分在各自浓度范围内与峰面积线性关系良好,相关系数r> 0.999,平均加样回收率99.28%~100.77%,RSD 0.69%~1.19%。结论:该方法专属性强,稳定性和重现性均较好,可为该药品的质量评价提供依据。Objective:To simultaneously determine chlorogenic acid,forsythoside A,aesculetin,genipo-side,baicalin,wogonoside and paeoniflorin in Lianqiao Baidu Pills by HPLC-DAD,and to compare their quality differences from different manufacturers.Methods:The samples were extracted with methanol and ultrasound for 40 min,injected in a Hypersil ODS-BP C18 column(250 mm×4.6 mm,5μm),and separated with the mobile phase comprising of 0.1%phosphoric acid-acetonitrile,flowing at 1.0 mL·min^(-1) in a gradi-ent elution manner at a temperature of 30℃;The injection volume was 15μL;The detection was based on multiwavelength switching in order of 326 nm(for chlorogenic acid),229 nm(for aesculetin,geniposide and paeoniflorin),330 nm(for forsythoside A and baicalin)and 275 nm(for wogonoside).Results:The seven constituents showed good linear relationships with their peak areas within respective concentration ranges with correlation coefficient r>0.999,whose average recoveries were 99.28%-100.77%with RSDs between 0.69%-1.19%.Conclusion:This method has strong specificity,good stability and reproducibility,and can be used for the quality evaluation of this drug.

关 键 词：连翘败毒丸 高效液相色谱法 二极管阵列检测器 含量测定 质量评价 

分 类 号：R927.2[医药卫生—药学]