黄瓜新矮化突变体的表型调查与转录组分析  

Phenotypic investigation and transcriptome analysis of a new dwarf mutant in cucumber

作　　者：陈明月 张梦瑶 郭梦雪 杨伟程 豆峻岭 杨路明[1] 杨森 CHEN Mingyue;ZHANG Mengyao;GUO Mengxue;YANG Weicheng;DOU Juning;YANG Luming;YANG Sen(College of Horticulture,Henan Agricultural University,Zhengzhou 450046,Henan,China)

机构地区：[1]河南农业大学园艺学院,郑州450046

出　　处：《中国瓜菜》2025年第3期18-26,共9页China Cucurbits And Vegetables

基　　金：国家自然科学基金(32172574);河南省高校科技创新人才支持计划项目(23HASTIT034);河南省优秀青年科学基金项目(222300420050)。

摘　　要：株高是影响黄瓜株型的重要性状。以获得的一个稳定遗传的黄瓜矮化自然突变体dw-37为研究材料,对已报道的黄瓜矮化基因的DNA序列进行克隆及序列比对,表明dw-37是一个新的黄瓜矮化突变体。对dw-37及正常株高的野生型黄瓜S-SB茎蔓的表型调查及细胞学观察,发现dw-37茎蔓的各个节间长度变短,茎蔓纵切面的细胞面积显著变小。dw-37和S-SB幼嫩茎蔓的转录组分析结果表明,多数差异表达基因在植物激素的合成及信号转导等通路中显著富集。综上,黄瓜矮化新突变体dw-37的矮化表型归因于茎节间细胞面积的减小,且这一过程受到多种植物激素的精细调控。研究结果为解析黄瓜株高调控机制提供了新的视角和遗传资源。Plant height is a crucial trait affecting the plant architecture of cucumber.A stably inherited natural dwarf mutant of cucumber,dw-37,was used as the experimental material.Cloning and sequence alignment of the DNA sequences of reported cucumber dwarfing genes indicated that dw-37 is a novel cucumber dwarf mutant.Phenotypic investigation and cytological observation of the stems and vines of dw-37 and the wild-type cucumber S-SB with normal plant height revealed that the length of each internode in the stems and vines of dw-37 was shortened,and the cell area in the longitudinal section of the stems and vines was significantly reduced.Transcriptome analysis of young stems and vines of dw-37 and S-SB showed that most of the differentially expressed genes were significantly enriched in pathways related to the synthesis and signal transduction of plant hormones.In summary,the dwarf phenotype of the novel cucumber dwarf mutant dw-37 is attributed to the reduction in cell area of the stem internodes,and this process is finely regulated by multiple plant hormones.These findings provide a new perspective and genetic resources for understanding the regulatory mechanism of cucumber plant height.

关 键 词：黄瓜 矮化 新突变体 转录组分析 激素 

分 类 号：S642.2[农业科学—蔬菜学]