外源S-亚硝基谷胱甘肽对NaCl胁迫下番茄幼苗PSⅡ活性和光保护能力的影响  

Effects of exogenous S-nitrosoglutathione on PSII activity and photoprotective capacity of tomato seedlings under NaCl stress

作　　者：朱晓玲 许静 刘慧英[1] ZHU Xiaoling;XU Jing;LIU Huiying(College of Agriculture,Shihezi University/Key Laboratory of Cultivation Physiology and Germplasm Resources Utilisation of Specialty Fruits and Vegetables,Xinjiang Production and Construction Corps,Shihezi 832003,Xinjiang,China)

机构地区：[1]石河子大学农学院·新疆生产建设兵团特色果蔬栽培生理与种质资源利用重点实验室,新疆石河子832003

出　　处：《中国瓜菜》2025年第3期94-105,共12页China Cucurbits And Vegetables

基　　金：新疆维吾尔自治区现代农业产业技术体系专项(XJARS-07);新疆维吾尔自治区重大科技专项(2022A02005-2)。

摘　　要：为探究外源S-亚硝基谷胱甘肽(GSNO)在缓解盐胁迫下番茄幼苗光抑制的光保护作用,以番茄品种中蔬四号为材料,研究100 mmol·L^(-1)NaCl胁迫下不同处理[番茄幼苗叶片分别喷施蒸馏水(对照)、0.1 mmol·L^(-1)GSNO、0.05 mmol·L^(-1)cPTIO、0.1 mmol·L^(-1)GSNO+0.05 mmol·L^(-1)cPTIO]对番茄幼苗生长指标、叶片色素含量、光化学效率、光能分配和活性氧(ROS)清除能力的影响。结果表明,0.1 mmol·L^(-1)GSNO在有效缓解NaCl胁迫、抑制番茄幼苗生长及净光合速率(Pn)的升高,同时伴随着叶绿素含量、最大PSⅡ和实际光化学效率(Fv/Fm和Y(Ⅱ))及PSⅡ光化学反应份额(P)分别显著提高了19.7%~31.4%、9.1%~10.3%、13.2%~51.8%和7.4%~44.6%,PSⅡ光能天线热耗散份额(D)、PSⅡ光能反应中心耗散份额(Ex)和双光系统间激发能分配不平衡偏离系数(β/α-1)分别显著降低了9.7%~14.1%、11.6%~34.8%和18.2%~53.9%。外源GSNO的施用也显著提高了盐胁迫下抗氧化酶活性,显著降低了丙二醛(MDA)含量和活性氧(ROS)水平。综上所述,0.1 mmol·L^(-1)GSNO提高了盐胁迫下番茄幼苗叶片叶绿素含量,优化了PSⅡ的光能分配及维持光系统间的激发能平衡分布、提高了PSⅡ光化学活性和抗氧化胁迫损伤能力,从而有效减轻了盐胁迫所致的光抑制程度和促进了番茄幼苗生长。In order to explore the photoprotective effect of exogenous GSNO on alleviating photoinhibition of tomato seed-lings under salt stress,the tomato variety Zhongshu No.4 was used as material to study the effects of different treat-ments(tomato seedling leaves sprayed with distilled water(control),0.1 mmol‧L^(-1) GSNO,0.05 mmol‧L^(-1) cPTIO,0.1 mmol‧L^(-1) GSNO+0.05 mmol‧L^(-1) cPTIO,respectively)on seedling growth,leaf pigment content,photochemical efficiency,light energy partitioning and reactive oxygen species(ROS)scavenging capacity under 100 mmol‧L^(-1) NaCl stress.The results showed that 0.1 mmol‧L^(-1) GSNO effectively alleviated the NaCl stress induced inhibition of tomato seedlings growth and net photosynthetic rate(Pn),while significantly increasing chlorophyll content,maximum and actual photochemical efficiency of PSII(Fv/Fm and Y(II))and PSII photochemical reaction share(P)by 19.7%-31.4%,9.1%-10.3%,13.2%-51.8%,and 7.4%-44.6%,respectively.The PSII photoenergy antenna heat dissipation share(D),the PSII photoenergy reaction center dissipation share(Ex),and the excitation energy distribution unbalanced deviation coef-ficient between the two optical systems(β/α-1)were significantly reduced by 9.7%-14.1%,11.6%-34.8%,and 18.2%-53.9%,respectively.The application of exogenous GSNO also significantly increased the activity of antioxidant enzymes under salt stress,and significantly decreased the levels of malondialdehyde(MDA)and reactive oxygen species(ROS).In summary,0.1 mmol‧L^(-1) GSNO increased the chlorophyll content of tomato seedlings leaves under salt stress,optimized the light energy distribution of PSII,maintained the balanced distribution of excitation energy between photosystems,improved the photochemical activity and antioxidant stress damage ability of PSII,thereby effectively reducing the degree of photoinhi-bition caused by salt stress and promoting tomato seedling growth.

关 键 词：番茄 S-亚硝基谷胱甘肽 NACL胁迫 光系统Ⅱ 光保护作用 

分 类 号：S641.2[农业科学—蔬菜学]