酚磺乙胺对基于Trinder反应原理的血清Cr、UA、TG的干扰性研究  

Interference study of etamsylat on serum Cr,UA and TG based on Trinder reaction principle

作　　者：姜艳[1] 桑国耀[1] 李辉[1] JIANG Yan;SANG Guoyao;LI Hui(Medical Laboratory Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,XinJiang 830054,China)

机构地区：[1]新疆医科大学第一附属医院医学检验中心,新疆乌鲁木齐830054

出　　处：《检验医学与临床》2025年第6期826-829,834,共5页Laboratory Medicine and Clinic

基　　金：省部共建中亚高发病成因与防治国家重点实验室项目(SKL-HIDCA-2023-25)。

摘　　要：目的基于Trinder反应原理探讨酚磺乙胺对血清肌酐(Cr)、尿酸(UA)、甘油三酯(TG)的干扰性,并评估酚磺乙胺对血清Cr、UA、TG检测结果的影响。方法参照美国临床和实验室标准协会公布的EP7-A3干扰试验指南,分别制备含Cr、UA、TG低水平和高水平的血清混合标本。以0.0、0.2 g/L分别作为酚磺乙胺最低、最高干扰剂量行配对差异实验,进而明确酚磺乙胺对上述3个项目在检测中的干扰性。同时,分别制备0.00、0.05、0.10、0.20 g/L的酚磺乙胺剂量,行剂量效应实验分析评估不同剂量酚磺乙胺在不同水平血清Cr、UA、TG中的干扰效能。结果0.2 g/L酚磺乙胺在低水平血清Cr、UA和TG的相对干扰率为-48.51%、-49.60%和-58.59%,对高水平血清Cr、UA和TG的相对干扰率为-44.90%、-45.44%和-43.94%;在0.2 g/L的酚磺乙胺干扰下,干扰组低、高水平血清Cr、UA和TG的测定结果约是对照组的一半。进一步的剂量效应实验结果显示,低、高水平Cr、UA和TG的测定结果均呈单调递减序列,即随酚磺乙胺剂量的升高,血清Cr、UA和TG在低、高水平检测的负干扰效应越大。结论酚磺乙胺对基于Trinder反应原理的血清Cr、UA、TG检测有负干扰效应,且其干扰效应随药物剂量的升高而增大。Objective To investigate the interference of etamsylate on serum creatinine(Cr),uric acid(UA)and triglyceride(TG)based on Trinder reaction principle,and to evaluate its influence on sample test results.Methods According to the EP7-A3 interference test guidelines of the American Clinical and Laboratory Standards Institute,serum mixed samples containing low levels and high levels of Cr,UA and TG were prepared,with 0 g/L as the lowest interference doses of etamsylate and 0.2 g/L as the highest interference doses of etamsylate,the paired difference experiment was performed to determine the interference of etamsylat on the above three items in the detection.At the same time,the concentrations of etamsylate(0,0.05,0.1 and 0.2 g/L)were prepared,and dose-effect experiments were performed to analyze and evaluate the interfering efficacy of different dose of etamsylatein in different levels of serum Cr,UA and TG.Results The relative interference rates of 0.2 g/L etamsylate was-48.51%,-49.60%and-58.59%in low level of Cr,UA and TG,and-44.90%,-45.44%and-43.94%in high level of Cr,UA and TG;under the interference of 0.2 g/L etamsylate,the determination results of low and high levels of serum Cr,UA and TG in the interference group were about half of those in the control group.Further dose-effect experiment results showed that the determination results of low and high levels of Cr,UA and TG showed a monotone decreasing sequence,that is,with the increase of the dose of etamsylate,the negative interference effect of serum Cr,UA and TG at low and high levels increased.Conclusion The detection of serum Cr,UA and TG based on Trinder reaction principle has negative interference effect,and the phase interference effect increases with the increase of drug dose.

关 键 词：酚磺乙胺 TRINDER反应 生化检验 剂量效应 药物干扰 

分 类 号：R446.1[医药卫生—诊断学]