精子DNA碎片率、高可染性指数及PARL与体外受精-胚胎移植妊娠结局的关系  

The relationship between sperm DNA fragmentation index,high DNA stainability,presenilin-associated rhomboid-like protein and pregnancy outcomes of in vitro fertilization-embryo transfer

作　　者：高雁 鲁洪岭 王琰[1] 郑加涛[1] 张伯阳 GAO Yan;LU Hongling;WANG Yan;ZHENG Jiatao;ZHANG Boyang(Department of Reproductive Medicine,Hebei Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine,Cangzhou 061000,Hebei,China;Department of Oncology,Hebei Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine,Cangzhou 061000,Hebei,China)

机构地区：[1]河北省沧州中西医结合医院生殖医学科,河北沧州061000 [2]河北省沧州中西医结合医院放化疗科,河北沧州061000

出　　处：《中国性科学》2025年第3期23-27,共5页Chinese Journal of Human Sexuality

基　　金：河北省中医药管理局2024年度中医药类科学研究课题项目(2024164)。

摘　　要：目的 探讨精子DNA碎片率(DFI)、高可染性指数(HDS)及早老素相关菱形样蛋白(PARL)与体外受精(IVF)-胚胎移植(ET)妊娠结局的关系。方法 选取2020年6月至2023年6月河北省沧州中西医结合医院收治的113例男性不育症患者作为研究对象,均采用IVF且在新鲜周期行ET,根据移植后配偶是否妊娠分为妊娠成功组(n=81)与妊娠失败组(n=32)。比较两组临床资料、精子DFI、HDS、PARL,采用Pearson法分析精子DFI、HDS、PARL与精子浓度、活力、畸形率的相关性,采用Logistic回归分析IVF-ET妊娠结局的影响因素,采用受试者工作特征(ROC)曲线分析精子DFI、HDS、PARL对IVF-ET妊娠结局的预测价值。结果 妊娠失败组优质胚胎率、精子浓度、活力、PARL低于妊娠成功组,精子畸形率、DFI、HDS高于妊娠成功组(P<0.05)。精子DFI、HDS与精子浓度、活力呈负相关,与精子畸形率呈正相关(P<0.05);PARL与精子浓度、活力呈正相关,与精子畸形率呈负相关(P<0.05)。精子DFI、HDS、PARL是IVF-ET妊娠结局的独立影响因素(P<0.05)。精子DFI、HDS、PARL联合预测IVF-ET妊娠结局优于各指标单独预测(P<0.05)。结论 不育症患者精子DFI、HDS、PARL与IVF-ET妊娠结局密切相关,可作为独立预测因子,联合预测性能较高。ObjectiveTo investigate the relationship between sperm DNA fragmentation index(DFI),high DNA stainability(HDS),presenilin-associated rhomboid-like protein(PARL)and pregnancy outcomes of in vitro fertilization(IVF)-embryo transfer(ET).MethodsA total of 113 male infertility patients admitted to the Hebei Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine from June 2020 to June 2023 were selected as the study objects.All patients underwent IVF and ET during the fresh cycle.They were divided into a successful pregnancy group(n=81)and a failed pregnancy group(n=32)based on whether their partners became pregnant after transfer.The clinical data,sperm DFI,HDS,and PARL of the two groups were compared.Pearson's method was used to analyze the correlation between sperm DFI,HDS,PARL and sperm concentration,motility,and malformation rate.Logistic regression analysis was used to investigate the influencing factors of IVF-ETpregnancy outcomes.Receiver operating characteristie(ROC)curve analysis was employed to assess the predictive value of sperm DFI,HDS,and PARL for IVF-ET pregnancy outcomes.I Results In the failed pregnancy group,the rate of high-quality embryos,sperm concentration,motility,and PARL were lower than those in the successful pregnancy group,while the sperm malformation rate,DFI,and HDS were higher than those in the successful pregnancy group(P<0.05).Sperm DFI and HDS were negatively correlated with sperm concentration and motility,but were positively correlated with sperm malformation rate(P<0.05).PARL was positively correlated with sperm concentration and motility,but was negatively correlated with sperm malformation rate(P<0.05).Sperm DFI,HDS,and PARL were independent factors affecting the pregnancy outcome of IVF-ET(P<0.05).The combined prediction of sperm DFI,HDS,and PARLfor IVF-ET pregnancy outcome was superior to the individual prediction of each indicator(P<0.05).Conclusions The sperm DFI,HDS,and PARL of infertility patients are closely related to the pregnancy outcome of IV

关 键 词：不育症 精子DNA碎片率 高可染性指数 早老素相关菱形样蛋白 体外受精-胚胎移植 妊娠结局 

分 类 号：R698[医药卫生—泌尿科学]