三七丹参片治疗非酒精性脂肪性肝病的作用机制研究  

Effect and mechanism of Sanqi danshen tablets in the treatment of non-alcoholic fatty liver disease

作　　者：雷雨田 冯丹 陈新利[1] 杨媛[1] 吴晖[1] LEI Yutian;FENG Dan;CHEN Xinli;YANG Yuan;WU Hui(Dept.of Clinical Pharmacy Center,the First Affiliated Hospital of Kunming Medical University,Kunming 650032,China)

机构地区：[1]昆明医科大学第一附属医院临床药学中心,昆明650032

出　　处：《中国药房》2025年第6期674-679,共6页China Pharmacy

基　　金：云南省科技厅科技计划项目基础研究专项(No.202301AT070152);云南省科技厅科技计划项目重大科技专项(生物医药)(No.2019ZF005);中华国际医学交流基金会-中华医学会临床药学分会临床药学科研基金项目(No.Z-202146-2101-2023)。

摘　　要：目的探讨三七丹参片治疗非酒精性脂肪性肝病(NAFLD)的潜在机制。方法采用网络药理学方法,挖掘三七丹参片治疗NAFLD的核心靶点,并进行基因本体(GO)功能及京都基因和基因组数据库(KEGG)通路富集分析。基于网络药理学结果,以SD大鼠为对象,以高脂饲料喂养构建NAFLD模型,考察三七丹参片对NAFLD大鼠肝组织脂滴空泡、脂质蓄积等病理改变以及脂质代谢、炎症反应、氧化应激指标的影响。结果共筛选出三七丹参片治疗NAFLD的核心靶点20个,主要涉及生物调节、细胞膜、结合等GO功能,并富集于炎症反应、氧化应激、脂质代谢相关信号通路。动物实验显示,与模型组相比,三七丹参片低、中、高剂量组和阳性对照药(辛伐他汀)组大鼠肝组织内脂滴空泡明显减少,脂滴数量明显减少且颜色变浅;其肝组织中总胆固醇、甘油三酯(三七丹参片中剂量组除外)、天冬氨酸转氨酶、丙氨酸转氨酶、肿瘤坏死因子α(三七丹参片低剂量组除外)、白细胞介素17(三七丹参片各剂量组除外)、丙二醛(三七丹参片低剂量组除外)含量均显著降低,超氧化物歧化酶含量均显著升高(P<0.01或P<0.05)。结论三七丹参片可通过影响炎症反应、氧化应激、脂质代谢等相关指标含量来发挥抗炎、抗氧化、调节脂质代谢的作用,从而改善大鼠的NAFLD。OBJECTIVE To investigate the potential mechanism of Sanqi danshen tablets in the treatment of non-alcoholic fatty liver disease(NAFLD).METHODS Core targets of Sanqi danshen tablets in the treatment of NAFLD were explored by network pharmacological methods.Gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were also performed.Based on the results obtained from network pharmacological studies,using SD rats as subjects,the NAFLD model was induced by feeding them high-fat diet.The effects of Sanqi danshen tablets on pathological changes such as lipid droplet vacuoles and lipid accumulation in the liver tissue of NAFLD rats,as well as its impact on relative indicators of lipid metabolism,inflammatory responses and oxidative stress,were investigated.RESULTS A total of 20 core targets for the treatment of NAFLD with Sanqi danshen tablets were screened,primarily involved in GO functions such as biological regulation,cellular membrane and binding,and enriched in signaling pathways related to inflammatory responses,oxidative stress and lipid metabolism.Compared with the model group,lipid droplet vacuoles were reduced significantly in low-dose,medium-dose,high-dose groups of Sanqi danshen tablets and positive control(simvastatin)group,the number of lipid droplets decreased significantly and the color became lighter.The contents of total cholesterol,triglyceride(except for mediumdose group of Sanqi danshen tablets),aspartate transaminase,alanine transaminase,tumor necrosis factor-α(except for low-dose group of Sanqi danshen tablets),interleukin-17(except for Sanqi danshen tablets groups)and malondialdehyde(except for lowdose group of Sanqi danshen tablets)in liver tissue were significantly decreased,while the content of superoxide dismutase was significantly increased(P<0.01 or P<0.05).CONCLUSIONS Sanqi danshen tablets exert anti-inflammatory,antioxidant and lipid metabolism regulating effects by influencing the levels of inflammation,oxidative stress

关 键 词：三七丹参片 非酒精性脂肪性肝病 炎症反应 氧化应激 脂质代谢 

分 类 号：R965[医药卫生—药理学] R285.5[医药卫生—药学]