双乙酰丙酮氧钒对人肾上腺皮质癌细胞增殖和侵袭的影响  

The effect of vanadyl bis(acetylacetonato)on the proliferation and invasion of human adrenocortical carci-noma cells

作　　者：甘美玉 吴春交 覃婧怡 罗佐杰[1] GAN Meiyu;WU Chunjiao;QIN Jingyi;LUO Zuojie(Department of Endocrinology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学第一附属医院内分泌科,广西南宁530021

出　　处：《实用医学杂志》2025年第6期781-789,共9页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:82260159)。

摘　　要：目的通过体外实验双乙酰丙酮氧钒[VO(acac)_(2)]对人肾上腺皮质癌SW-13、NCl-H295R细胞系的作用,了解VO(acac)_(2)是否对肾上腺皮质癌细胞的增殖、迁移和侵袭具有促进或抑制作用。方法取对数生长期的SW-13和NCI-H295R细胞,用6.25、12.5、25、50、75、100、200μmol/L的VO(acac)_(2)分别干预SW-13、NCI-H295R细胞24和48 h,米托坦作为阳性对照组。CCK-8(cell counting kit-8)检测VO(acac)_(2)对SW-13、NCI-H295R细胞活力的影响;后用0、6.25、12.5、25μmol/L的VO(acac)_(2)分别干预SW-13、NCI-H295R细胞48 h,流式细胞术检测VO(acac)_(2)对SW-13、NCI-H295R细胞凋亡的影响;划痕实验检测VO(acac)_(2)对SW-13、NCI-H295R细胞迁移能力的影响;Transwell实验检测VO(acac)_(2)对SW-13、NCI-H295R细胞侵袭能力的影响;克隆实验检测VO(acac)_(2)对SW-13、NCI-H295R细胞增殖能力的影响。结果CCK-8结果显示VO(acac)_(2)呈时间和浓度依赖性抑制SW-13、NCI-H295R细胞的活力,VO(acac)_(2)对SW-13细胞作用24及48 h的半数抑制浓度(half-maximal inhibitory concentration,IC_(50))分别为(62.98±6.67)、(14.61±1.66)μmol/L,对NCI-H295R作用24及48 h的IC50分别为(46.78±7.89)、(12.61±2.98)μmol/L。流式细胞术结果显示VO(acac)_(2)呈浓度依赖性促进SW-13、NCI-H295R细胞的凋亡(P<0.05)。划痕实验结果示随着VO(acac)_(2)干预浓度增加,SW-13、NCI-H295R细胞的迁移率随之下降(P<0.05)。Transwell实验结果示VO(acac)_(2)可呈浓度依赖性抑制SW-13、NCI-H295R细胞的侵袭能力。克隆实验结果显示VO(acac)_(2)可呈浓度依赖性抑制SW-13、NCI-H295R细胞的增殖能力。结论VO(acac)_(2)可抑制人肾上腺皮质癌细胞SW-13、NCI-H295R的增殖、迁移和侵袭能力,诱导其凋亡。Objective To investigate the effects of bis(acetylacetonato)oxovanadium(IV)[VO(acac)_(2)]on human adrenocortical carcinoma cell lines SW-13 and NCI-H295R in vitro,aiming to determine whether VO(acac)_(2)promotes or inhibits the proliferation,migration,and invasion of these cells.Methods SW-13 and NCI-H295R cells in logarithmic growth phase were exposed to VO(acac)_(2)at concentrations of 6.25,12.5,25,50,75,100,and 200μmol/L for 24 and 48 hours,respectively.Mitotane served as the positive control.Cell viability was assessed using the CCK-8 assay to evaluate the effects of VO(acac)_(2)on SW-13 and NCI-H295R cells.Subsequently,cells were treated with VO(acac)_(2)at concentrations of 0,6.25,12.5,and 25μmol/L for 48 hours,and flow cytometry was employed to investigate the impact of VO(acac)_(2)on apoptosis.The migratory ability of the cells was evaluated using a wound healing assay,while their invasive capacity was assessed via a Transwell assay.Additionally,the clonogenic assay was used to determine the proliferative potential of SW-13 and NCI-H295R cells following VO(acac)_(2)treatment.Results The CCK-8 results demonstrated that VO(acac)_(2)inhibited the viability of SW-13 and NCIH295R cells in a time-and concentration-dependent manner.Specifically,the half-maximal inhibitory concentrations(IC50)for VO(acac)_(2)against SW-13 cells were 62.98±6.67μmol/L after 24 hours and(14.61±1.66)μmol/L after 48 hours of treatment,while the corresponding IC_(50)values for NCI-H295R cells were 46.78±7.89μmol/L and 12.61±2.98μmol/L,respectively.Flow cytometry analysis revealed that VO(acac)_(2)induced apoptosis in both SW-13 and NCI-H295R cells in a concentration-dependent manner(P<0.05).The wound healing assay indicated a significant reduction in the migratory rate of SW-13 and NCI-H295R cells with increasing concentrations of VO(acac)_(2)(P<0.05).Transwell assay results showed that VO(acac)_(2)significantly inhibited the invasive ability of SW-13 and NCI-H295R cells in a concentration-dependent fashion.Finally,the clonog

关 键 词：双乙酰丙酮氧钒 肾上腺皮质癌 增殖 侵袭 凋亡 

分 类 号：R586.9[医药卫生—内分泌]