UPLC-MS/MS法同时测定银黄胶囊中10种成分的含量  

Simultaneous determination of 10 components in Yinhuang capsules by UPLC-MS/MS

作　　者：何枭宇 孔虎林 赵平华 陈洁 方静 王启砚 HE Xiao-yu;KONG Hu-lin;ZHAO Ping-hua;CHEN-Jie;FANG-Jing;WANG Qi-yan(Jingmen Food and Drug Quality Inspection Institute,Jingmen Hubei 448000;Hubei BiocauseHeilen Pharmaceutical Co.,Ltd.,Jingmen Hubei 448000)

机构地区：[1]荆门市食品药品质量检验所,湖北荆门448000 [2]湖北亨迪药业股份有限公司,湖北荆门448000

出　　处：《中南药学》2025年第2期514-518,共5页Central South Pharmacy

基　　金：湖北省药品监督管理局2020—2021年度科研项目(No.20200115)。

摘　　要：目的建立UPLC-MS/MS法同时测定银黄胶囊中6个有机酸类(绿原酸、新绿原酸、隐绿原酸、3,5-O-二咖啡酰基奎宁酸、3,4-O-二咖啡酰基奎宁酸、4,5-O-二咖啡酰基奎宁酸)和4个黄酮类(黄芩苷、黄芩素、汉黄芩苷、汉黄芩素)成分的含量。方法采用Hypersil GOLD VANQUISH C_(18)柱(2.1 mm×100 mm,1.9μm),以乙腈-0.2%甲酸水溶液为流动相,梯度洗脱,柱温35℃,流速为0.3 mL·min^(-1);质谱采用电喷雾离子源,以多反应监测模式进行定量分析。结果10种成分在一定浓度范围内与峰面积线性关系良好(r>0.9990);平均加样回收率(n=3)均在97.35%~99.26%,RSD在0.36%~0.83%。6批银黄胶囊中上述10种成分含量分别为6.981~12.56、0.8929~2.084、1.407~3.454、0.5611~0.9415、0.8823~1.647、1.984~3.465、94.16~135.2、1.768~3.214、7.985~10.06、6.487~10.11 mg·g^(-1)。结论该方法快速准确,稳定灵敏,可用于银黄胶囊的质量控制。Objective To simultaneously determine 6 organic acids(chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,3,5-O-dicaffeoyl quinic acid,3,4-O-dicaffeoyl quinic acid and 4,5-O-dicaffeoyl quinic acid)and 4 flavonoids(baicalin,baicalein,wogonoside and wogonin)in Yinhuang capsules by UPLC-MS/MS.Methods The separation was performed on an Hypersil GOLD VANQUISH C_(18)(2.1 mm×100 mm,1.9μm).The mobile phase consisted of acetonitrile-0.2%formic acid by gradient elution at the column temperature of 35℃.The flow rate was 0.3 mL·min^(-1).Electrospray ionization source was used for the quantitative analysis in multiple reaction monitoring modes.Results Ten components showed good linearity with all correlation coefficients>0.9990.The average recoveries(n=3)ranged from 97.35%to 99.26%with RSD from 0.36%to 0.83%.The contents of the above 10 components in the 6 batches of Yinhuang capsules were within 6.981~12.56,0.8929~2.084,1.407~3.454,0.5611~0.9415,0.8823~1.647,1.984~3.465,94.16~135.2,1.768~3.214,7.985~10.06 and 6.487~10.11 mg·g^(-1),respectively.Conclusion The method is simple,accurate,reliable and sensitive for the quality control of Yinhuang capsules.

关 键 词：银黄胶囊 有机酸类 黄酮类 含量测定 UPLC-MS/MS 

分 类 号：R917[医药卫生—药物分析学] R284.1[医药卫生—药学]