青藏高原东北部不同海拔小麦种子内生真菌多样性  

Fungal diversity in wheat seeds at different altitudes in the northeastern Qinghai-Xizang Plateau

作　　者：吴芳 杨晨艺 张远鹏 先梦娜 王僖苗 鲜豪 潘志芬[3] 蒲青青 WU Fang;YANG Chenyi;ZHANG Yuanpeng;XIAN Mengna;WANG Ximiao;XIAN Hao;PAN Zhifen;PU Qingqing(Faculty of Quality Management and Inspection&Quarantine,Yibin University,Yibin 644000,China;Sichuan Higher Education Engineering Research Center for Agri-Food Standardization and Inspection,Yibin University,Yibin 644000,China;Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610213,China;College of Food Science,Southwest University,Chongqing 400715,China)

机构地区：[1]宜宾学院质量管理与检验检测学部,宜宾644000 [2]宜宾学院农食产品标准化与检验检测四川省高等学校工程研究中心,宜宾644000 [3]中国科学院成都生物研究所,成都610213 [4]西南大学食品科学学院,重庆400715

出　　处：《应用与环境生物学报》2024年第6期1222-1229,共8页Chinese Journal of Applied and Environmental Biology

基　　金：第二次青藏高原综合科学考察研究项目(2019QZKK0303);农食产品标准化与检验检测四川省高等学校工程研究中心开放基金项目(24NSYJZX03);宜宾学院启航项目(2019QD14);国家级大学生创新创业训练计划项目(202410641050)资助。

摘　　要：为揭示小麦内生真菌对不同海拔的响应,利用高通量测序技术探究青藏高原东北部不同海拔小麦种子内生真菌的结构组成和多样性.结果表明:12个小麦种子样本共获得895542条序列,聚类到1021个分类操作单元(operational taxonomic units,OTUs),分别属于8个门、33个纲、80个目、169个科、311个属、534个种.不同海拔组间α多样性指数差异不显著(P>0.05),群落结构总体上看,门水平上以子囊菌门(Ascomycota,92.44%)为主,属水平上优势属为链格孢菌属(Alternaria)、附球菌属(Epicoccum)、Lasionectria和枝孢属(Cladosporium),由于青藏高原海拔差异较大,小麦种子内生真菌群落物种在不同海拔间存在显著差异,低海拔组优势菌属是链格孢菌属,相对丰度78.91%,中海拔组优势菌属为附球菌属(37.10%)、枝孢属(17.66%)、链格孢菌属(16.82%),高海拔组优势菌属为Lasionectria(34.00%)、Paraconiothyrium(12.15%)、曲霉属(Aspergillus,10.77%)和枝孢属(10.08%);β多样性分析表明低海拔组样品间相似度较高,中海拔和高海拔组样品间相似度较低并且有交叉.各海拔组均有自己的生物标记,其中高海拔组最多,有7种,中海拔组次之,有5种,低海拔组最少,仅有3种.FUNGuild功能预测结果显示,小麦种子内生真菌包括植物病原菌、动物病原菌、木材腐生菌、地衣寄生菌、土壤腐生菌等功能菌群.可见,青藏高原东北部地区小麦种子内生真菌种类丰富,内生真菌多样性受海拔影响不显著,群落组成受到海拔影响显著,内生真菌中包括潜在的有益功能菌属和多种种传病害病原菌;这些结果为青藏高原小麦种子内生真菌的开发利用和小麦种传病害的防治提供了理论依据.In this study,we used high-throughput sequencing technology to examine the structural composition and diversity of wheat endophytic fungi at different elevations in the northeastern region of the Qinghai-Xizang Plateau.We accordingly obtained 895542 sequences from 12 wheat seed samples,which could be clustered into 1021 taxonomic operating units(OTUs),categorized into to eight phyla,33 classes,80 orders,169 families,311 genera,and 534 species.In terms ofαdiversity indices,we detected no significant differences among samples collected at different elevations.At the phylum level,Ascomycota species(92.44%)were identified as the predominant fungal community,whereas Alternaria,Epicoccum,Lasionectria,and Cladosporium were found to be the predominant genera.In addition,we detected significant elevational differences with respect to the composition of endophytic fungal communities,among which Alternaria,with a relative abundance of 78.91%;were identified a dominant fungi in the low-altitude group;Epicoccum(37.10%),Cladosporium(17.66%),and Alternaria(16.82%)were predominant in the mid-altitude group;and Lasionectria(34.00%),Paraconiothyrium(12.15%),Aspergillus(10.77%)and Cladosporium(10.08%)predominated in the high-altitude group.Analysis ofβdiversity indicated a high similarity among the samples in the low-altitude group,whereas the mid-and highaltitude groups were found to be characterized by lower similarity and crossover.Furthermore,for each of the assessed elevation groups,we identified distinct biomarkers,with seven,five,and three such markers being detected in the high-,mid-,and low-altitude groups,respectively.The results obtained for FUNGuild function prediction revealed that among the different fungal functional groups,wheat seed endophytes included plant pathogens,animal pathogens,wood saprophytes,lichen parasites,and soil saprophytes.Collectively,our findings revealed that although the diversity of endophytic fungi in the northeastern region of the Qinghai-Xizang Plateau is not significantly influenced by el

关 键 词：小麦 内生真菌 多样性分析 海拔 

分 类 号：S512.1[农业科学—作物学]