大豆苗期耐盐性种质资源鉴定与指标筛选  

Identification and Screening of Salt Tolerance Indexes in Soybean Germplasm Resources at Seedling Stage

作　　者：韩岱 时晓磊 吴玉成 田蕊 丁孙磊 山其米克[1] 赵靓 张恒斌[3] 战勇[3] 刘兵强[4] 严勇亮[1] HAN Dai;SHI Xiaolei;WU Yucheng;TIAN Rui;DING Sunlei;SHAN Qimike;ZHAO Liang;ZHANG Hengbin;ZHAN Yong;LIU Bingqiang;YAN Yongliang(Institute of Crop Germplasm Resources,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Agricultural College of Xinjiang Agricultural University,Urumqi 830052,China;Crop Research Institute,Xinjiang Academy of Agricultural Reclamation Sciences,Shihezi Xinjiang 832000,China;Institute of Grain and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050035,China)

机构地区：[1]新疆农业科学院农作物品种资源研究所,乌鲁木齐830091 [2]新疆农业大学农学院,乌鲁木齐830052 [3]新疆农垦科学院作物研究所,新疆石河子832000 [4]河北省农林科学院粮油作物研究所,石家庄050035

出　　处：《西北农业学报》2025年第3期446-457,共12页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：新疆维吾尔自治区重大科技专项(2022A03004);国家重点研发计划(2023YFD2300105);新疆农业科学院自主培育项目“农作物种质资源繁殖与利用团队”(nkyzztd-004);财政部和农业农村部国家现代农业产业技术体系(CARS-04-CES27);新疆维吾尔自治区天山创新团队“大豆高产抗逆育种与超高产栽培创新团队”。

摘　　要：挖掘苗期耐盐性强的大豆种质资源,筛选出大豆耐盐表型鉴定指标,为大豆耐盐品种选育提供优异亲本材料,并为大豆耐盐性进一步研究提供科学依据。采用盆栽法,以浓度1.5%NaCl溶液模拟盐胁迫,对102份大豆种质资源进行苗期耐盐性鉴定评价,测定株高、茎粗、茎节数等9个性状指标,计算耐盐系数,使用相关性分析、主成分分析法、隶属函数法等方法对大豆苗期耐盐性进行综合评价,以逐步回归分析法为依据建立大豆种质资源苗期耐盐性最优回归方程,并筛选耐盐性评价最优指标。结果表明:盐胁迫对各指标均有不同程度的抑制;通过综合评价可知102份大豆材料苗期耐盐性存在差异,按照耐逆性强弱可划分为5类;进一步利用逐步回归的方法构建大豆苗期耐盐性评价模型D=-0.486+Sdm×0.224+Ncl×0.456+Sfm×0.174+Nsn×0.387+St×0.259(Sdm、Ncl、Sfm、Nsn和St分别代表地上部分干质量、复叶数、地上部分鲜质量、茎节数和茎粗的耐盐系数),筛选出5个耐盐性鉴定指标。The aim of this study to screen elite soybean germplasm with strong salt tolerance at the seedling stage,providing superior parental materials for breeding salt-tolerant soybean varieties and a scientific basis for further research.Using a pot experiment,102 soybean germplasm resources were evaluated for salt tolerance under a 1.5%NaCl solution,simulating saline stress.Nine traits,including plant height(Ph),stem thickness(St),number of stem node(Nsn),number of compound leaves(Ncl),leaf length(Ll),leaf width(Lw),petiole length(Pl),shoot fresh mass(Sfm)and shoot dry mass(Sdm)were determined.The salt tolerance coefficient was calculated,and the soybean salt tolerance was comprehensively evaluated using correlation analysis,principal component analysis and membership function methods.The results showed that salt stress inhibited all measured traits to some degrees.Comprehensive evaluation revealed significant variation in salt tolerance among the 102 soybean germplasm resources,which were classified into five categories based on their stress tolerance.A salt tolerance evaluation model D=-0.486+Sdm×0.224+Ncl×0.456+Sfm×0.174+Nsn×0.387+St×0.259 was developed,where Sdm,Ncl,Sfm,Nsn and St represent the salt tolerance coefficient of shoot dry mass,compound leaf number,shoot fresh mass,number of stem node,and stem thickness,respectively.Five optimal salt tolerance indices were identified based on this model.

关 键 词：大豆 苗期 盐胁迫 指标筛选 

分 类 号：S51[农业科学—作物学]