苹果李属坏死环斑病毒检测及其遗传多样性分析  

Detection of Prunus Necrotic Ringspot Virus and Analysis of Its Genetic Diversity

作　　者：王骆凡 张桃霞 李运龙 宋浩祺 陈雅寒 WANG Luofan;ZHANG Taoxia;LI Yunlong;SONG Haoqi;CHEN Yahan(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学植物保护学院,兰州730070

出　　处：《西北农业学报》2025年第3期527-534,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：甘肃农业大学人才启动金(GAU-KYQD-2019-22);国家自然科学基金(32160628);甘肃省自然科学基金(21JR7RA820);甘肃省高等学校创新基金(2021B-117)。

摘　　要：为了明确李属坏死环斑病毒(prunus necrotic ringspot virus,PNRSV)在甘肃省苹果部分产区的发生及遗传变异情况,对采自5个地区表现花叶症状和无明显症状的93份苹果叶片样品进行检测,通过RT-PCR在天水和兰州苹果样品中检测到PNRSV。分别克隆了5个PNRSV分离物的外壳蛋白(coat protein,CP)基因。序列分析结果表明,5个PNRSV分离物间核苷酸和氨基酸序列一致率分别为97.3%~99.4%和95.5%~99.1%。5个PNRSV分离物与NCBI数据库中其他21个PNRSV分离物的核苷酸和氨基酸序列一致率分别为91.5%~100%和89.6%~100%。利用PNRSV CP基因序列构建了系统发育树,26个PNRSV分离物共分成3个组,本研究中的5个PNRSV分离物与美国分离物AF013287聚集在一起。基因交流分析表明,桃(Prunus persica)和巴旦木(P.dulcis)的PNRSV分离物种群之间存在频繁的基因交流,桃(P.persica)和西梅(P.cerasifera)分离物之间容易发生遗传漂变。To elucidate the occurrence and genetic variability of prunus necrotic ringspot virus(PNRSV)in selected apple-producing regions of Gansu Province,93 apple leaf samples with mosaic symptoms and asymptomatic samples were collected from 5 distinct areas.PNRSV was detected in Tianshui and Lanzhou apple samples by RT-PCR.The coat protein(CP)genes of five PNRSV isolates were cloned and subsequently sequenced.Sequence analysis revealed that the nucleotide and amino acid sequence identities among the five PNRSV isolates ranged from 97.3%to 99.4%and 95.5%to 99.1%,respectively.Compared with 21 additional PNRSV isolates from the NCBI database indicated nucleotide and amino acid sequence identities ranging from 91.5%to 100%and 89.6%to 100%,respectively.The phylogenetic tree constructed using the CP gene sequence of PNRSV categorized the 26 PNRSV isolates into three distinct groups.The five PNRSV isolates from this study clustered together with the American isolate AF013287,suggesting potential phylogenetic relationships.Gene exchange analysis indicated frequent genetic interactions among PNRSV isolates from peach(Prunus persica)and almond(P.dulcis)populations,while genetic drift was more likely to occur readily between isolates from peach and prune(P.cerasifera).

关 键 词：苹果病毒 PNRSV 遗传多样性 基因交流 

分 类 号：S43[农业科学—农业昆虫与害虫防治]