盐胁迫对不同品种水稻根际土壤细菌多样性与结构的影响  

Effects of Salt Stress on Bacteria Diversity and Structure of Rhizosphere Soil in Different Rice Varieties

作　　者：周玉杰 贺治洲[1] 胡伟 林秋云[1] 林越辉 龙开意[1] 谢振宇[1] ZHOU Yujie;HE Zhizhou;HU Wei;LIN Qiuyun;LIN Yuehui;LONG Kaiyi;XIE Zhenyu(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院热带作物品种资源研究所,海南海口571101

出　　处：《热带作物学报》2025年第3期754-763,共10页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.323RC533,No.320RC727);海南省水稻产业技术体系“种质资源创制与品种选育岗”(No.HNARS-RICE)

摘　　要：盐分是制约沿海滩涂盐渍化土地利用的主要因素之一,水稻(Oryza sativa L.)是盐渍化土地利用的首选粮食作物,而某些根际微生物可以提高水稻的抗盐能力。本研究选取不同品种水稻在NaCl胁迫下进行全生育期培育,采用Illumina Miseq测序技术,测定并分析不同品种水稻根际土壤细菌群落多样性和结构,旨在筛选适宜盐渍化土地种植的水稻品种。结果表明:就根际土壤细菌群落多样性而言,0.6%NaCl处理下,B3和B6品种的物种丰富度显著高于B1品种。根际土壤细菌群落结构方面,不同浓度NaCl处理下,B1、B3和B6品种的相对丰度大于10%的优势细菌门均为绿弯菌门(Chloroflexi)、变形菌门(Proteobacteria)和放线菌门(Actinobacteriota);不同品种水稻根际土壤细菌群落结构存在差异,富集的物种数量和种类也不同,0.6%NaCl处理下B6品种富集了14个细菌类群分支,如酸杆菌门(Acidobacteriae)、念珠藻科(Nostocaceae)、Vicinamibacterales、热微菌目(Thermomicrobiales)、KD4-96等。因此,沿海滩涂盐渍化土地种植B6品种水稻有利于根际土壤优势细菌群落的形成。Salt is a main factor limiting the utilization of saline land in coastal beach.Rice is the preferred food crop for the utilization of salinized land,and some rhizosphere microorganisms can improve the salt resistance of rice.In order to screen suitable rice varieties planted in salinized land,different rice varieties were selected to cultivate under NaCl stress during the whole growth period,and the diversity and structure of bacterial communities in rhizosphere soil were measured and analyzed by Illumina Miseq sequencing technology.The bacterial community diversity in the rhizosphere soil,the species richness of varieties B3 and B6 were significant higher than those of variety B1 in 0.6%NaCl treat-ments.The community structure,the dominant bacterial phyla were Chloroflexi,Proteobacteria and Actinobacteria with relative abundance more than 10%of three varieties under different concentrations of NaCl treatments.There were dif-ferences in the bacterial community structure of rhizosphere soil,and the number and types of enriched species were also different in different rice varieties.Variety B6 enriched fourteen bacterial taxa branches under 0.6%NaCl treatments,such as Acidobacteriae,Nostocaceae Vicinamibacterales,Thermomicrobiales,KD4-96,etc.Therefore,B6 planted in salinized land of coastal mudflat is beneficial to the formation of dominant bacterial community in rhizosphere soil.

关 键 词：水稻 盐胁迫 根际土壤 细菌群落 16S rRNA 

分 类 号：S511[农业科学—作物学] S154.3