高效液相色谱法测定几种药食同源中药材中苯并(a)芘  

Determination of BAP in Chinese Medicinal Materials of the Same Origin as Food and Medicine by High Performance Liquid Chromatography

作　　者：赵超群 陶瑞 舒楠茜 梁晶晶 ZHAO Chaoqun;TAO Rui;SHU Nanxi;LIANG Jingjing(Zhejiang Institute for Food and Drug Control,Key Laboratory of Quality and Safety of Functional Food for State Market Regulation&Key Laboratory of Zhejiang Marketing Bureau(Key Laboratory of Health Food Quality and Safety&Zhejiang Key Laboratory of Biopharmaceutical Contact Materials),Hangzhou 310052,China)

机构地区：[1]浙江省食品药品检验研究院,国家市场监管重点实验室(功能食品质量安全)&浙江省市场局重点实验室(保健食品质量安全重点实验室&浙江省生物医药接触材料重点实验室),浙江杭州310052

出　　处：《云南化工》2025年第3期66-69,共4页Yunnan Chemical Technology

基　　金：浙江省药品监管科技计划项目(2023004);浙江省市场监督管理局雏鹰计划核心项目(CY2022227)。

摘　　要：利用分子印迹固相萃取-全自动固相萃取技术结合高效液相色谱法建立测定几种药食同源中药材中并(a)芘的方法。在粉碎后的试样中加入正己烷进行提取,利用苯并(a)芘分子印迹柱-全自动固相萃取仪富集、净化,采用岛津Inertsustain C_(18)(250 mm×∅4.6 mm,5μm)色谱柱,以乙腈-水(体积比85∶15)为流动相进行洗脱,外标法定量计算。结果表明,苯并(a)芘质量浓度在0.4004~20.02 ng/mL范围内线性关系良好,相关系数为0.9994,检出限为0.15μg/kg,定量限为0.40μg/kg。方法回收率为88.5%~102.5%,精密度(n=6)均小于4.12%。建立的方法灵敏度高,操作简便,准确度好的同时实现了仪器自动化。A method fordetermination of benzo(a)pyrene was established,which was relied on automatic solid phase extraction-high performance liquid chromatography.The crushed sample was extracted by adding n-hexane,enriched and purified by benzo(a)pyrene molecular imprinting column-automatic solid-phase extractor,Shimadzu Inertsustain C_(18)(250 mm×∅4.6 mm,5μm)column was used and elutied with acetonitrile-water(85∶15).External standard method was used for quantitative analysis.The results showed that the linear ranges of BAP were 0.4004 to 20.02ng/mL and the correlation coefficients was 0.9994.The limits of detection was 0.15μg/kg,and the limits of quantification was 0.40μg/kg respectively.The recoveries were between 88.5%-102.5%with the relative standard deviations were less than 4.12%.The method has the characteristics of high sensitivity,good recovery rate and good precision.It realized the automation of the instrument and simplified the operation steps.

关 键 词：苯并(A)芘 药食同源中药材 高效液相色谱 全自动固相萃取 分子印迹 荧光检测器 

分 类 号：R284[医药卫生—中药学]