金银花挥发油抗流感病毒作用研究  

Study on Anti-Influenza Virus Effect of Volatile Oil from Lonicerae Japonicae Flos

作　　者：徐小雯 XU Xiaowen(TCM Pharmacy,Chun’an First People’s Hospital,Hangzhou,Zhejiang 311700,China)

机构地区：[1]淳安县第一人民医院中药房,浙江杭州311700

出　　处：《生物医学工程学进展》2025年第1期56-61,共6页Progress in Biomedical Engineering

基　　金：杭州市医药卫生科技项目(OO20190178)。

摘　　要：目的研究金银花挥发油抗流感病毒的作用。方法将不同浓度的金银花挥发油注入鸡胚的尿囊腔中,收集尿囊液样本并行血凝实验。根据不同浓度组的血凝抑制滴定结果,评估金银花挥发油在鸡胚中对流感病毒的抑制效能。以滴鼻方式将甲型流感病毒小鼠肺适应株A/PR/8/34(H1N1)滴入乙醚轻度麻醉的小鼠鼻中,构建小鼠流感病毒性肺炎模型。将不同浓度的金银花挥发油通过预防给药,根据肺指数研究其抗流感病毒的作用。结果不同浓度组金银花挥发油均能明显降低感染流感病毒鸡胚尿囊液中的血凝滴度(P<0.01),并且对小鼠流感病毒性肺炎具有显著的治疗作用。结论金银花挥发油对甲型流感病毒的增殖有明显的抑制作用,同时能通过缓解流感病毒性肺炎症状发挥抗流感的作用。Objective To study the anti-influenza virus effects of volatile oil of Lonicerae Japonicae Flos.Methods Different concentrations of Lonicerae Japonicae Flos volatile oil were injected into the allantoic cavity of chicken embryos,and allantoic fluid samples were collected for haemagglutination test.Based on the results of haemagglutination inhibition titration in different concentration groups,the inhibitory efficacy of the volatile oil of Lonicerae Japonicae Flos against influenza virus in chicken embryos was evaluated.A mouse lung adapted strain A/PR/8/34(H1N1)of influenza A virus was dropped into the noses of mice with mildly anesthetised to establish a mouse model of influenza pneumonia.Different concentrations of volatile oil of Lonicerae Japonicae Flos were given prophylactic administration to study its anti-influenza virus effect by lung index.Results The volatile oil of Lonicerae Japonicae Flos could significantly reduce the hemagglutination titer in allantoic fluid of chicken embryos infected with influenza virus(P<0.01),and had a significant easing effect on influenza virus pneumonia in mice.Conclusion The volatile oil of Lonicerae Japonicae Flos has an obvious inhibitory effect on the proliferation of influenza A virus,and at the same time can exert an anti-influenza virus effect by alleviating the symptoms of influenza viral pneumonia.

关 键 词：金银花 挥发油 流感病毒 血凝实验 流感病毒性肺炎 

分 类 号：R285.5[医药卫生—中药学]