双侧卵巢切除模型大鼠潮热特征的初步探索及二仙汤的调节作用  

A Preliminary Study on the Characteristics of Hot Flushes in Bilateral Ovariectomy Rats and the Regulating Effect of Erxian Decoction

作　　者：谢海纳 潘志强[1] 曹琳娜 XIE Haina;PAN Zhiqiang;CAO Linna(School of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学中医学院,上海201203

出　　处：《南京中医药大学学报》2025年第3期323-332,共10页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(82374307)。

摘　　要：目的探索双侧卵巢切除术后大鼠的潮热特征以及二仙汤的作用。方法48只雌性SD大鼠随机分为假手术组、双侧卵巢切除组、17β雌二醇组和二仙汤组,共4组,每组12只,采用双侧卵巢切除术去势后分别灌胃17β雌二醇(0.1 mg·kg^(-1)·d^(-1))、二仙汤(8 g·kg^(-1)·d^(-1)),持续6周。在造模第21天和第42天,检测大鼠体质量、肛温、体表红外热像图等表征信息。ELISA检测血清E_(2)含量;采用HE染色观察大鼠子宫和足垫汗腺的病理变化;免疫组化检测下丘脑雌激素受体(ER)及温度感受器瞬时受体电位香草酸亚型3(TRPV3)、瞬时受体电位香草酸亚型4(TRPV4)表达;Western blot检测ERβ、PAC1R和PKA蛋白表达。结果与假手术组同期比较,双侧卵巢切除组大鼠体质量增长显著(P<0.01)而17β雌二醇组和二仙汤组大鼠体质量增长缓慢。与假手术组相比,双侧卵巢切除组和17β雌二醇组造模后第21天肛温显著上升(P<0.01),造模后第42天肛温显著下降(P<0.05);造模后第21天与第42天,双侧卵巢切除组、17β雌二醇组和二仙汤组大鼠体表最高温度均显著下降(P<0.01),造模第42天,双侧卵巢切除组大鼠尾部/体内温度比值显著升高(P<0.05);双侧卵巢切除组汗点明显增加(P<0.01);双侧卵巢切除组、17β雌二醇组和二仙汤组子宫指数均呈明显下降(P<0.01);双侧卵巢切除组血清E_(2)显著下降(P<0.05);双侧卵巢切除组大鼠下丘脑ER阳性细胞数显著减少(P<0.01),而TRPV3阳性细胞显著增多(P<0.05);17β雌二醇组ER和TRPV3阳性细胞数减少(P<0.05),TRPV4阳性细胞数增加(P<0.01);17β雌二醇抑制PAC1R和PKA蛋白表达(P<0.05),二仙汤显著抑制PAC1R、PKA和ERβ蛋白表达(P<0.01)。与双侧卵巢切除组相比,二仙汤组第21天肛温显著下降(P<0.01);17β雌二醇组和二仙汤组汗点明显减少(P<0.01);二仙汤组E_(2)水平显著升高(P<0.05),下丘脑ER阳性细胞数显著增多(P<0.01),ERβ和PAC1R蛋白表达量明显下�OBJECTIVE To explore the characteristics of hot flushes and the effect of Erxian Decoction in rats after bilateral oophorectomy.METHODS Forty-eight female SD rats were randomly divided into 4 groups:sham operation group,bilateral ovariectomy group,17β-estradiol treatment group and Erxian Decoction treatment group,with 12 rats in each group.After bilateral ovariectomy,rats were gavaged with 17β-estradiol(0.1 mg·kg^(-1)·d^(-1))and Erxian Decoction(8 g·kg^(-1)·d^(-1))for 6 weeks.On the 21st and 42nd days of modeling,the body weight,rectal temperature,infrared thermography of the body surface and other characteristic information of the rats were detected.Serum E_(2)content was detected by ELISA.The pathological changes of the uterus and foot pad sweat glands of the rats were observed by HE staining.The hypothalamic estrogen receptor(ER)and thermoreceptor transient receptor potential vanilloid 3(TRPV3)and transient receptor potential vanilloid 4(TRPV4)were detected by immunohistochemistry.The protein expression of ERβ,PAC1R and PKA was detected by Western blot.RESULTS Compared with the sham operation group during the same period,the body weight of the rats in the bilateral ovariectomy group increased significantly(P<0.01),while the body weight of the rats in the 17β-estradiol group and Erxian Decoction group increased slowly.Compared with the sham operation group,the rectal temperature of the bilateral ovariectomy group and the 17β-estradiol group increased significantly on the 21st day after modeling(P<0.01),and decreased significantly on the 42nd day after modeling(P<0.05);on the 21st and 42nd days after modeling,the maximum body surface temperature of the rats in the bilateral ovariectomy group,the 17β-estradiol group and the Erxian Decoction group decreased significantly(P<0.01),and on the 42nd day after modeling,the tail/body temperature ratio of the rats in the bilateral ovariectomy group increased significantly(P<0.05);the number of sweat points in the bilateral ovariectomy group increased significant

关 键 词：二仙汤 双侧卵巢切除术 温度 潮热 肾上腺皮质 雌二醇 

分 类 号：R285.5[医药卫生—中药学]