基于UHPLC-Q Exactive Orbitrap-MS分析酸枣仁汤化学成分及雌性大鼠入血成分  

Analysis of Chemical Components of Suanzaoren Decoction and Components Absorbed into Blood of Female Rats Based on UHPLC-Q Exactive Orbitrap-MS

作　　者：孔子洋 吴亮[2] 理文 韩杰 盛晨敏 练宇恒 孟令栋 赵玉男[5] 卞尧尧 KONG Ziyang;WU Liang;LI Wen;HAN Jie;SHENG Chenmin;LIAN Yuheng;MENG Lingdong;ZHAO Yunan;BIAN Yaoyao(School of Acupuncture-Moxibustion and Tuina,School of Health Preservation and Rehabilitation,Nanjing University of Chinese Medicine,Nanjing 210023,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Faculty of Chinese Medicine,Macao University of Science and Technology,Macao 999078,China;Hemodialysis Department of Yangzhou Hospital of Traditional Chinese Medicine,Yangzhou 225002,China;School of Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Provincial Engineering Research Center of Traditional Chinese Medicine External Medication Development and Application,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学针灸推拿学院·养生康复学院,江苏南京210023 [2]南京中医药大学药学院,江苏南京210023 [3]澳门科技大学中医药学院,中国澳门999078 [4]扬州市中医院血透室,江苏扬州225002 [5]南京中医药大学医学院,江苏南京210023 [6]南京中医药大学江苏省中医外用药开发与应用工程研究中心,江苏南京210023

出　　处：《南京中医药大学学报》2025年第3期375-382,共8页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81603529);江苏省中医药科技发展计划重点项目(ZD202404);南京中医药大学自然科学基金项目(XZR2023028)。

摘　　要：目的利用超高效液相色谱-串联质谱(UHPLC-Q Exactive Orbitrap-MS)技术,分析酸枣仁汤灌胃给药后的入血成分。方法以SD雌性大鼠为实验对象,灌胃给予酸枣仁汤,收集血清样品,采用UHPLC-Q Exactive Orbitrap-MS技术分析酸枣仁汤水提物和血清并鉴定吸收入血的原型成分和代谢产物,并与LuMet-TCM数据库进行比对分析。结果酸枣仁汤水提物共鉴定出458个成分,入血成分共鉴定出26个化学成分,其中23个为原型成分,3个为代谢产物。结论研究发现的入血原型成分可能是酸枣仁汤的有效成分,为酸枣仁汤的药效物质基础研究提供参考。OBJECTIVE To analyze the blood components of Suanzaoren Decoction after oral administration using UHPLC-Q Exactive Orbitrap-MS technology.METHODS Female Sprague-Dawley(SD)rats were used as experimental subjects,and Suanzaoren Decoction was administered orally.Serum samples were collected,and the aqueous extract of Suanzaoren Decoction and the serum were analyzed using UHPLC-Q Exactive Orbitrap-MS technology to identify the prototype components and metabolites absorbed into the blood by comparing and analyzing with the LuMet-TCM database.RESULTS It showed that a total of 458 components were identified in the aqueous extract of Suanzaoren Decoction,and 26 chemical components were identified in the blood,including 23 prototype components and 3 metabolites.CONCLUSION The prototype components absorbed into the blood discovered in this study may be the active ingredients of Suanzaoren Decoction,providing a reference for the research on the pharmacodynamic material basis of Suanzaoren Decoction.

关 键 词：酸枣仁汤 雌性大鼠 入血成分 超高效液相色谱-串联质谱 代谢产物 

分 类 号：R274.1[医药卫生—中医骨伤科学]