广西生姜镰刀菌枯萎病病原菌的分离鉴定  

Isolation and Identification of the Pathogen Causing Ginger Fusarium Wilt in Guangxi

作　　者：杨维超 李小莉 文俊丽[3] 黄皓[3] 周生茂[3] 袁高庆[1] YANG Wei-chao;LI Xiao-li;WEN Jun-li;HUANG Hao;ZHOU Sheng-mao;YUAN Gao-qing(College of Agriculture,Guangxi University,Nanning 530004,PRC;Lechang Branch,Guangdong Tobacco Shaoguan City Co.,Ltd.,Lechang 512200,PRC;Vegetable Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,PRC)

机构地区：[1]广西大学农学院,广西南宁530004 [2]广东烟草韶关市有限公司乐昌市分公司,广东乐昌512200 [3]广西农业科学院蔬菜研究所,广西南宁530007

出　　处：《湖南农业科学》2025年第2期54-58,共5页Hunan Agricultural Sciences

基　　金：广西重点研发计划(桂科AB21238002);广西农业科学院基本科研业务专项(2015YZ03;桂农科2024YP082)。

摘　　要：为确定引起广西生姜镰刀菌枯萎病的病原菌种类,研究采集了广西生姜主产区疑似镰刀菌枯萎病的生姜病株,采用组织分离法分离纯化获得16株病原菌菌株,结合形态学观察和TEF-1α序列分析对其进行鉴定,并接种3株典型菌株的分生孢子悬浮液进行致病性测定。结果表明:16株菌株均与尖孢镰刀菌(Fusarium oxysporum)聚为一支,经柯赫氏法则验证,明确了尖孢镰刀菌是引起广西生姜镰刀菌枯萎病的主要病原菌。This study aims to identify the pathogen causing ginger Fusarium wilt in Guangxi.The plant samples with suspected ginger Fusarium wilt were collected from main ginger-producing areas in Guangxi.A total of 16 pathogen strains were isolated by the tissue culture method and identified by morphological observation and TEF-1αsequence analysis.The pathogenicity of three typical strains was determined by inoculating the conidial suspensions.The results showed that all the 16 isolates were clustered with Fusarium oxysporum in the phylogenetic tree and their pathogenicity was confirmed according to Koch's postulates.In conclusion,F.oxysporum was the main pathogen causing ginger Fusarium wilt in Guangxi.

关 键 词：生姜镰刀菌枯萎病 病原菌鉴定 尖孢镰刀菌 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]