DNA分子鉴定和理化分析相结合对灵芝质量评价的研究与应用  

Research and Application of Quality Evaluation of Ganoderma lucidum Based on the Combination of DNA Molecular Identification and Physicochemical Analysis

作　　者：张庆英[1] 董岩岩[1] ZHANG Qingying;DONG Yanyan(Liaocheng Vocational and Technical College,Liaocheng 252000 Shandong,China)

机构地区：[1]聊城职业技术学院,山东聊城252000

出　　处：《广州中医药大学学报》2025年第4期988-991,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：山东省中医药重点科技项目(编号:Z-2022041);聊城市重点研发计划项目(政策引导类)(编号:2022YDSF87);聊城市哲学社会科学规划“中医药文化传承发展研究”专项课题(编号:ZXKT2024343);聊城职业技术学院重点科技项目(编号:2022LZYK02,2022LZYK01)。

摘　　要：【目的】应用DNA鉴定和理化分析相结合对20批灵芝进行质量评价。【方法】对20批灵芝进行DNA提取、聚合酶链反应(PCR)扩增、纯化、测序、输入中药材DNA条形码鉴定系统,进行鉴定。再进行标志性成分含量测定。【结果】1、2、3、4、5、6、9、10、11、12、13、14、15、16、18、20号灵芝为药典赤芝品种,而7、8、17、19号灵芝为非药典赤芝品种;对16批赤芝进行含量测定,含量符合药典规定有15个品种,含量由高到低的顺序为:2、1、5、10、18、3、4、16、15、11、13、14、6、9、12号。其中20号样品低于规定,其含量为0.38%。【结论】DNA分子鉴定和理化分析相结合能快速进行灵芝真伪优劣的质量评价。Objective The quality evaluation of 20 batches of Ganoderma was carried out by the combination of DNA identification and physicochemical analysis.Methods The 20 batches of Ganoderma were identified by DNA extraction,polymerase chain reaction(PCR)amplification,purification,sequencing,and inputting the Chinese medicines into DNA barcode identification system.Then the content determination of marker components was carried out.Results Ganoderma of No.1,2,3,4,5,6,9,10,11,12,13,14,15,16,18 and 20 belonged to Ganoderma lucidum(Leyss.ex Fr.)Karst.in the Pharmacopoeia,while No.7,8,17 and 19 did not belong to Ganoderma lucidum(Leyss.ex Fr.)Karst.in the Pharmacopoeia.Content determination was conducted on 16 batches of Ganoderma lucidum(Leyss.ex Fr.)Karst.,in which 15 batches were up to the standards of Pharmacopoeia,the decreasing sequence of contents was as follows:No.2,1,5,10,18,3,4,16,15,11,13,14,6,9,and 12,and No.20 was failed to meet the standards,with a content of 0.38%.Conclusion The combination of DNA molecular identification and physicochemical analysis can be used for rapid quality evaluation of the authenticity and superiority of Ganoderma.

关 键 词：灵芝 DNA分子鉴定 理化分析 质量评价 

分 类 号：R282.5[医药卫生—中药学]