朴树种子内源抑制物的研究  

Study on the Endogenous Inhibitors in the Seeds of Celtis sinensis

作　　者：黄莹[1] 高捍东[1] 高榕 沈思凡 Huang Ying;Gao Handong;Gao Rong;Shen Sifan(College of Forestry and Grassland,College of Soil and Water Conservation,Nanjing Forestry University/Southern Tree Seed Inspection Center,National Forestry and Grassland Administration/Co−Innovation Center for the Sustainable Forestry in Southern China,Nanjing Jiangsu 210037,China)

机构地区：[1]南京林业大学林草学院,水土保持学院,国家林业和草原局南方林木种子检验中心,南方现代林业协同创新中心,江苏南京210037

出　　处：《西南林业大学学报(自然科学)》2025年第2期11-19,共9页Journal of Southwest Forestry University:Natural Sciences

基　　金：国家林业和草原局国家级成果推广项目(2020133138)资助。

摘　　要：采用系统溶剂法对朴树种子种胚及种壳的浸提液分别分离出石油醚相、乙醚相、乙酸乙酯相、甲醇相,并利用白菜种子的发芽试验检测该5个相的生物活性,同时结合气相色谱-质谱(GC-MS)联用仪和生物测定,探讨抑制物与朴树种子休眠之间的关系。结果表明:朴树种子各分离相均对白菜种子发芽存在一定的抑制作用,其中以乙酸乙酯相和乙醚相的抑制性最强,其次分别是甲醇相和石油醚相。GC-MS测定和生物检测结果表明,朴树种子内源抑制物成分主要为有机酸(醋酸、棕榈酸)、酯类物质(邻苯二甲酸二甲酯)以及一些苯类物质(丁基化羟基甲苯)。The petroleum ether phase,ether phase,ethyl acetate phase and methanol phase were separated from the extract of seed embryo and seed shells of Celtis sinensis seeds by systematic solvent method,and the biological activity of these 5 phases was detected by the germination test of cabbage seeds.At the same time,gas chromatography-mass spectrometry(GC-MS)combined with bioassay,were used to explore the relationship between the inhibitors and seed dormancy.The results showed that each separated phase of C.sinensis seeds had a certain inhibitory effect on the germination of cabbage seeds,among which ethyl acetate phase and ether phase were the strongest,followed by methanol phase and petroleum ether phase,respectively.Combined with the results of GC-MS and biological detection,it was found that the main components of endogenous inhibitors were organic acids:acetic acid,palmitic acid,and ester substances:dimethyl phthalate.And some benzene substances:butylated hydroxytoluene,may also have an inhibitory effect.

关 键 词：朴树 种子休眠 内源抑制物 GC-MS 

分 类 号：S792[农业科学—林木遗传育种]