萱草叶片大小相关基因挖掘与分析  

Exploration and Analysis of Leaf Size Related Genes of Hemerocallis fulva

作　　者：陶桂祥 杨帆[1] 严苓方 姚国琼 李伟 孙正海[1] Tao Guixiang;Yang Fan;Yan Lingfang;Yao Guoqiong;Li Wei;Sun Zhenghai(Yunnan International Joint R&D Center for Intergrated Utilization of Ornamental Grass/International Technological Cooperation Base of High Effective Economic Forestry cultivating of Yunnan Province/South and Southeast Asia Joint R&D Center of Economic Forest Full Industry Chain of Yunnan Province/College of Landscape Architecture and Horticulture,Southwest Forestry University,Kunming Yunnan 650233,China;Yun-nan Technological College,Kunming Yunnan 650224,China;Yunnan Jicheng Landscape Technology Co.Ltd.,Honghe Yunnan 652339,China)

机构地区：[1]西南林业大学云南省观赏草集成利用国际联合研发中心,云南省高效经济林培育示范型国际科技合作基地,云南省面向南亚东南亚经济林全产业链联合研发中心,园林园艺学院,云南昆明650233 [2]云南林业职业技术学院,云南昆明650224 [3]云南吉成园林科技股份有限公司,云南红河652339

出　　处：《西南林业大学学报(自然科学)》2025年第2期58-65,共8页Journal of Southwest Forestry University:Natural Sciences

基　　金：云南省教育厅科学研究基金项目(2019Y0152)资助;云南省重点研发计划项目(202202AE090012-04)资助。

摘　　要：为挖掘控制萱草叶片大小基因,对不同叶片大小的2个萱草品种进行了转录组测序和组装,构建了转录组文库,并通过对差异基因进行通路分析和Pathway功能注释。结果显示:共得到90 366条基因簇(unigene),涉及10 671条表达量差异显著的unigene,其中表达量下调的基因序列有4 188条(39.25%),上调的基因有6 483条(60.75%);将10 671条unigene与NCBI中已报道的与叶片大小相关的678个基因进行比对,并结合参考文献,共得到17个可能与控制萱草叶片大小相关的基因序列,并进一步结合qRT-PCR证实了ARF2、BRI1、CESA3、CYP90、IAA4共5个基因在萱草叶片发育过程中与叶片大小密切相关,且发挥着重要作用。To investigate the genes controlling leaf size of Hemerocallis fulva,the study conducted transcriptome sequencing and assembly on 2 varieties of H.fulva with different leaf sizes.A transcriptome library was constructed,followed by pathway analysis and functional annotation of differentially expressed genes.The findings revealed that a total of 90,366 gene clusters(unigenes),among which 10,671 unigenes showed significant differences in expression quantity.These unigenes accounted for 39.25%of the genetic sequences,with 6,483 genes upregulated(60.75%).By comparing these unigenes with 678 genes in NCBI database that are associated with leaf size and integrating references,the study identified 17 gene sequences that may control leaf size.Further confirmation through qRT-PCR revealed that ARF2,BRI1,CESA3,CYP90,IAA4 are closely associated with leaf development in H.fulva and play important roles in determining leaf size.

关 键 词：萱草 叶片大小 转录组测序 基因 

分 类 号：S682[农业科学—观赏园艺]