参附芎泽方对异丙肾上腺素诱导大鼠心肌纤维化的影响  

Effects of Shenfu Xiongze Prescription on isoproterenol induced myocardial fibrosis in rats

作　　者：王雪晴 钟伟 李彩红[2] 于远望[1] Wang Xueqing;Zhong Wei;Li Caihong;Yu Yuanwang(School of Basic Medicine,Shaanxi University of Chinese Medicine,Xianyang 712000,China;Department of Cardiology,the Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000,China)

机构地区：[1]陕西中医药大学基础医学院,咸阳712000 [2]陕西中医药大学第二附属医院心内科,咸阳712000

出　　处：《国际中医中药杂志》2025年第3期336-341,共6页International Journal of Traditional Chinese Medicine

基　　金：陕西省科技厅自然科学基础研究计划(2023-JC-QN-0975、2023-JC-YB-726);咸阳市重点研发计划项目(L2022ZDYFSF015);陕西省教育厅项目(20JK0589);陕西省中西医结合心血管病防治重点实验室项目(303-1730202030)。

摘　　要：目的探讨参附芎泽方对异丙肾上腺素诱导大鼠心肌纤维化的影响。方法将SD大鼠按随机数字表法分为空白对照组、模型组及参附芎泽方低、中、高剂量组,每组7只。除空白对照组外,其余各组采用腹腔注射异丙肾上腺素建立心肌纤维化大鼠模型。参附芎泽方低、中、高剂量组造模同时灌胃参附芎泽方溶液8.4、16.8、33.6 g/kg,空白对照组和模型组灌胃等体积生理盐水,1次/d,连续3周。采用超声心动图观察心脏结构和功能,ELISA法检测血清脑利尿钠肽(BNP)、IL-6水平,HE、Masson染色观察心肌组织病理变化,Western blot法检测心肌组织中TGF-β1、α-平滑肌肌动蛋白(α-SMA)蛋白表达。结果与模型组比较,参附芎泽方各剂量组射血分数、左室短轴缩短率降低(P<0.01),左心室舒张末期内径、左心室收缩末期内径升高(P<0.05或P<0.01),心肌组织TGF-β1、α-SMA蛋白表达降低(P<0.01或P<0.05);参附芎泽方中、高剂量组大鼠血清BNP、IL-6水平降低(P<0.05或P<0.01),参附芎泽方低剂量组大鼠血清IL-6水平降低(P<0.01)。结论参附芎泽方可抑制异丙肾上腺素诱导的大鼠心肌纤维化,改善大鼠心功能,其作用机制可能与减轻心脏炎症、下调TGF-β1、α-SMA蛋白表达有关。Objective To investigate the effects of Shenfu Xiongze Prescription on myocardial fibrosis induced by isoproterenol(ISO)in rats.Methods SD rats were divided into blank control group,model group,and Shenfu Xiongze Prescription low-,medium-,and high-dosage groups according to random number table method.with 7 rats in each group.Except for the blank control group,the other groups were induced to form myocardial fibrosis in rats by intraperitoneal injection of isoprenaline.Shenfu Xiongze Prescription low-,medium-,and high-dosage groups were administered Shenfu Xiongze Prescription solution at dosages of 8.4,16.8,and 33.6 g/kg,respectively,while the blank control group and the model group were given the same volume of normal saline for gavage,once a day,for consecutive 3 weeks.The heart structure and function were observed by echocardiography,the levels of BNP and IL-6 in serum were detected by ELISA,the pathological changes of myocardial tissue were observed by HE and Masson staining,and the expressions of TGF-β1 andα-SMA proteins in myocardial tissue were detected by Western blot.Results Compared with the model group,the EF and FS values of Shenfu Xiongze Prescription in all dosage groups decreased(P<0.01),the LVIDd and LVIDs increased(P<0.05 or P<0.01),and the expressions of TGF-β1 andα-SMA proteins in myocardial tissue decreased(P<0.01 or P<0.05);the levels of BNP and IL-6 in serum of the Shenfu Xiongze Prescription medium and high-dosage groups decreased(P<0.05 or P<0.01),while the level of IL-6 in the low-dosage group decreased(P<0.01).Conclusion Shenfu Xiongze Prescription can inhibit rat myocardial fibrosis induced by isoprenaline,improve rat cardiac function,and its mechanism may be related to reducing inflammation in the heart and down-regulating the expressions of TGF-β1 andα-SMA proteins.

关 键 词：心力衰竭 心肌纤维化 异丙肾上腺素 参附芎泽方 大鼠 

分 类 号：R54[医药卫生—心血管疾病]