逍遥聪耳方预处理对肝郁脾虚型耳鸣模型大鼠的影响  

Effects of Xiaoyao Conger Decoction pretreatment on model rats of tinnitus with syndrome of liver-qi stagnation and spleen deficiency

作　　者：贾志姣 张滢颖 吴文丽 王俊杰[1] 李静波[1] Jia Zhijiao;Zhang Yingying;Wu Wenli;Wang Junjie;Li Jingbo(Department of Otolaryngology,Henan Province Hospital of Traditional Chinese Medicine,Otolaryngology Institute of Henan University of Chinese Medicine,Zhengzhou 450003,China;Department of Otolaryngology,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Graduate School,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]河南省中医院耳鼻喉科河南中医药大学耳鼻咽喉研究所,郑州450003 [2]北京中医药大学东方医院耳鼻喉科,北京100078 [3]北京中医药大学研究生院,北京100029

出　　处：《国际中医中药杂志》2025年第2期184-189,共6页International Journal of Traditional Chinese Medicine

基　　金：河南省中医药科学研究专项课题(2022ZYZD10)。

摘　　要：目的探究逍遥聪耳方预处理对肝郁脾虚型耳鸣模型大鼠的影响及其作用机制。方法将大鼠按随机数字表法分为空白组、模型组、阳性组及逍遥聪耳方高、低剂量组,每组7只。除空白组外,其余各组大鼠采用复合病因法制备肝郁脾虚型耳鸣模型。阳性组灌胃卡马西平15 mg/kg,逍遥聪耳方高、低剂量组灌胃逍遥聪耳方水煎液30、15 g/kg,空白组和模型组灌胃等体积的生理盐水,1次/d,持续4周。于给药前及给药2、4周记录大鼠条件反射逃避行为次数;于给药前、后进行中医证候量化评分;监测4、12、20、28 kHz频率下大鼠听性脑干反应(ABR)阈值,采用实时荧光定量PCR检测下丘、耳蜗IL-1βmRNA水平,液质联用法测定海马组织γ-氨基丁酸(GABA)、谷氨酸(Glu)水平。结果模型组大鼠可观察到烦躁易怒、毛发枯黄、舌质深红等肝郁证候及精神萎靡、活动减少、粪便稀溏等脾虚证候。与模型组比较,逍遥聪耳方低、高剂量组大鼠跳台逃避行为次数减少(P<0.05或P<0.01),中医证候量化评分降低(P<0.05或P<0.01),在12、20、28 kHz频率下的ABR阈值降低(P<0.01),下丘、耳蜗IL-1βmRNA水平降低(P<0.05或P<0.01),海马组织Glu水平降低(P<0.01)。结论逍遥聪耳方预处理可改善肝郁脾虚型耳鸣大鼠的耳鸣症状,其作用机制可能与其改善情绪障碍和应激状态,减轻神经炎症反应,调控中枢神经递质平衡,纠正听觉中枢兴奋性过高等有关。Objective To explore the effects of Xiaoyao Conger Decoction pretreatment on model rats of tinnitus with syndrome of liver-qi stagnation and spleen deficiency(TLSSD)and its preliminary mechanisms.Methods The rats were divided into four groups:a blank group,a model group,a positive group,and Xiaoyao Conger high-and low-dosage groups,with 7 rats in each group according to random number table method.Except for the blank group,the TLSSD model was prepared by the composite etiological method in all groups of rats.The positive group received a gavage of carbamazepine at 15 mg/kg,while Xiaoyao Conger high-and low-dosage groups were given 30 g/kg and 15 g/kg of Xiaoyao Conger Decoction,respectively.The blank and model groups were administered an equal volume of saline once daily for 4 weeks.The frequency of conditioned avoidance response behaviors in rats was recorded before and at 2 and 4 weeks post-drug administration;TCM syndrome scores were documented before and after treatment;auditory brainstem response(ABR)thresholds were monitored at frequencies of 4,12,20,and 28 kHz;IL-1βmRNA expression in the inferior colliculus and cochlea was detected by RT-qPCR;and the levels ofγ-aminobutyric acid(GABA)and glutamic acid(Glu)in hippocampal tissues were determined by LC-MS/MS.Results The model group exhibited symptoms of liver depression,including irritability,yellow fur,and a dark red tongue,along with signs of spleen deficiency such as lethargy,decreased activity,and loose stools.Compared to the model group,rats in the Xiaoyao Conger high-and low-dosage groups demonstrated a reduction in conditioned avoidance response behaviors(P<0.05 or P<0.01),a decrease in TCM syndrome scores(P<0.05 or P<0.01),lower ABR thresholds at 12,20,and 28 kHz(P<0.01),reduced levels of IL-1βmRNA in the inferior colliculus and cochlea(P<0.05 or P<0.01),and decreased hippocampal Glu levels(P<0.01).Conclusion The therapeutic effect of Xiaoyao Conger Decoction pretreatment on TLSSD rats may be related to its ability to improve the mood disorders and

关 键 词：耳鸣 肝郁脾虚 白细胞介素1Β Γ-氨基丁酸 谷氨酸 逍遥聪耳方 大鼠 

分 类 号：R28[医药卫生—中药学]