壮医经筋推拿对神经病理性疼痛大鼠去泛素化酶BRCC3去泛素化修饰的影响及镇痛机制研究  

Study on the effects of meridian massage of Zhuang medicine on deubiquitination modification of BRCC3 in neuropathic pain rats and its analgesic mechanism

作　　者：伍思琦 郑胜 罗晟文 关嘉恒 何育风[2] 梁英业 甘炜[2] Wu Siqi;Zheng Sheng;Luo Shengwen;Guan Jiaheng;He Yufeng;Liang Yingye;Gan Wei(Graduate School of Guangxi University of Chinese Medicine,Nanning 530000,China;Tuina Department of the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530000,China)

机构地区：[1]广西中医药大学研究生院,南宁530000 [2]广西中医药大学第一附属医院推拿科,南宁530000

出　　处：《国际中医中药杂志》2025年第2期190-195,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(82205305);广西自然科学基金(2020GXNSFBA297053);广西中医药适宜技术开发与推广项目(GZSY22-26);广西中医药重点研究室——广西中医药痛症桂派推拿干预重点研究室(2024年)。

摘　　要：目的探讨壮医经筋推拿对神经病理性疼痛(NPP)模型大鼠去泛素化酶BRCA1/BRCA2的复杂亚基3(BRCC3)的去泛素化修饰及其镇痛作用机制。方法将大鼠按随机数字表法分为正常组、假手术组、模型组、假推拿组和壮医经筋推拿组,每组9只。除正常组和假手术组外,其余各组制备脊神经结扎模型。术后第1天开始对壮医经筋推拿组、假推拿组进行干预,持续干预14 d,其余3组不予干预;于术前0 d及术后1、7、14 d分别测定各组大鼠机械性刺激缩足阈值(PWMT)和热痛缩足反应潜伏期(PWTL),采用Western blot法检测脊髓组织BRCC3、NOD样受体热蛋白结构域相关蛋白3(NLRP3)蛋白表达,采用ELISA法检测血清IL-1β水平。结果与模型组比较,术后7、14 d壮医经筋推拿组PWMT、PWTL升高(P<0.05),脊髓组织NLPR3、BRCC3表达降低(P<0.05),血清IL-1β水平降低(P<0.05);与假推拿组比较,术后7、14 d壮医经筋推拿组PWMT、PWTL升高(P<0.05),脊髓组织中NLRP3蛋白表达降低(P<0.05),血清IL-1β水平降低(P<0.05)。结论壮医经筋推拿可减轻脊神经结扎模型大鼠痛敏反应,其机制与抑制去泛素化酶BRCC3表达,升高NLRP3泛素化水平,阻碍NLRP3活化,从而阻断炎症因子介导的免疫炎症反应有关。Objective To explore the deubiquitination modification of the deubiquitinase BRCC3 in rats with neuropathic pain(NPP)treated with meridian tuina of Zhuang medicine and its analgesic molecular mechanism.Methods Rats were divided into normal group,sham-operation group,model group,sham-tuina group,and meridian tuina of Zhuang medicine group using a random number table method,with 9 rats in each group.Except for the normal and sham operation groups,spinal nerve ligation models were prepared in all other groups.On the first day after surgery,intervention was carried out on the meridian tuina of Zhuang medicine and sham-tuina groups for 14 days,while the other three groups were not intervened;the paw withdrawal mechanical threshold(PWMT)and paw withdrawal thermal latency(PWTL)of each group of rats were measured at 0 days before operation,1,7,and 14 days after operation.Western blot was used to detect the expressions of BRCC3 and NLRP3 proteins in spinal cord tissue,and ELISA was used to detect the level of IL-1βin serum.Results On postoperative 7 and 14 d,compared with the model group,the meridian tuina of Zhuang medicine group showed an increase in PWMT and PWTL,a decrease in NLPR3 and BRCC3 expression in spinal cord tissue,and a decrease in serum IL-1βlevels(P<0.05).Compared with the sham-tuina group,the meridian tuina of Zhuang medicine group showed an increase in PWMT and PWTL,a decrease in NLRP3 protein expression in spinal cord tissue,and a decrease in IL-1βlevels in serum(P<0.05).Conclusion Meridian tuina of Zhuang medicine can alleviate pain sensitivity in SNL model rats,and its mechanism is related to the inhibition of the expression of deubiquitinase BRCC3 by meridian massage of Zhuang medicine,which increases the ubiquitination level of NLRP3 and hinders its activation,thereby blocking the immune inflammatory response mediated by inflammatory factors.

关 键 词：镇痛 壮医经筋推拿 神经病理性疼痛 去泛素化 大鼠 

分 类 号：R29[医药卫生—民族医学]