僵蚕及其炮制品核苷类成分差异性研究  

Study on the difference in nucleoside composition of Bombyx Batryticatus and its processed products

作　　者：邱韵静 吴妙梨 童培珍 邓立萍 杨丽 李国卫 Qiu Yunjing;Wu Miaoli;Tong Peizhen;Deng Liping;Yang Li;Li Guowei(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司广东省中药配方颗粒企业重点实验室,佛山528244

出　　处：《国际中医中药杂志》2025年第2期213-220,共8页International Journal of Traditional Chinese Medicine

基　　金：国家工信部2022年产业技术基础公共服务平台项目-中药全产业链质量技术服务平台项目(2022-230-221);2022年佛山市南海区重点领域科技攻关专项(南科〔2023〕20号-18)。

摘　　要：目的建立可同时测定僵蚕及其炮制品中6个成分含量的HPLC特征图谱方法,为其质量评价提供依据。方法色谱柱为Waters Xselect HSS T3(250 mm×4.6 mm,5μm),流动相为乙腈-5 mmol/L乙酸铵溶液(用醋酸溶液调整pH值至4.5),梯度洗脱,流速1.0 ml/min,柱温30℃,检测波长260 nm,进样量为10μl。采用SIMCA-P 14.0软件进行聚类分析和PLS-DA,评价僵蚕及其炮制品质量。结果僵蚕及炒僵蚕HPLC特征图谱中确定9个共有峰,指认出7个共有峰。聚类分析和PLS-DA可僵蚕与炒僵蚕聚为2类,其中峰4、峰2、峰1、峰6、峰3为其质量差异标志物。尿酸、次黄嘌呤、尿苷、腺嘌呤、鸟苷、腺苷分别在43.95~879.04、2.05~41.07、2.35~47.09、2.02~40.36、2.32~46.43、2.32~46.43μg/ml内线性关系良好(R2≥0.9999),精密度、稳定性(24 h)、重复性RSD均低于3.00%,平均加样回收率在94.57%~102.01%,RSD均在0.96%~3.32%。15批僵蚕样品中,尿酸、次黄嘌呤、尿苷、腺嘌呤、鸟苷、腺苷平均含量依次为11.51、0.33、0.38、0.42、0.35、0.36 mg/g,15批炒僵蚕样品上述6个成分含量依次为12.06、0.35、0.36、0.18、0.21、0.24 mg/g。结论本研究建立的方法简便、快捷、重复好,PLS-DA可将僵蚕及其炮制品进行区分,可为僵蚕及其炮制品的质量评价提供参考。Objective To establish an HPLC characteristic chromatogram method to simultaneously determine the content of 6 components in Bombyx Batryticatus and its processed products;To provide a scientific means for quality evaluation.Methods The HPLC analysis was performed on a Waters Xselect HSS T3 column(250 mm×4.6 mm,5μm),with a mobile phase consisting of acetonitrile and 5 mmol/L ammonium acetate solution(adjust pH to 4.5 with acetic acid)in gradient mode at a flow rate of 1.0 ml/min.The column temperature was 30℃.The detection wavelength was 260 nm.The injection volume was 10μl.The SIMCA-P 14.0 software was used for cluster analysis and partial least squares discriminant analysis to evaluate the quality of Bombyx Batryticatus and its processed products.Results Nine common peaks were identified in the HPLC characteristic spectra of Bombyx Batryticatus and its processed products,and seven common peaks were identified.Cluster analysis and partial least squares discriminant analysis can classify Bombyx Batryticatus and its processed products into two groups,with peak 4,peak 2,peak 1,peak 6,and peak 3 as their mass difference markers.Uric Acid,Hypoxanthine,Uridine,Adenine,Guanosine,and Adenosine showed good linearity(R2≥0.9999)within 43.95-879.04,2.05-41.07,2.35-47.09,2.02-40.36,2.32-46.43,2.32-46.43μg/ml,respectively.The precision,stability(24 h),repeatability relative standard deviation(RSD)were all<3.00%.The average spiked recoveries were all in the range of 94.57%-102.01%,and the RSDs were all in the range of 0.96%-3.32%.In 15 batches of Bombyx Batryticatus,the content of the above 6 components,was determined to be 11.51,0.33,0.38,0.42,0.35,0.36 mg/g in the following order.And in 15 batches of fried Bombyx Batryticatus,the content of components was determined to be 12.06,0.35,0.36,0.18,0.21,0.24 mg/g in the following order.Conclusions The method established in this study is simple,rapid,and repeatable.PLS-DA can distinguish Bombyx Batryticatus and its processed products,which can provide a reference for the qu

关 键 词：僵蚕 炮制(中药) 特征图谱 偏最小二乘法判别分析 

分 类 号：R28[医药卫生—中药学]