金毛耳草药材UPLC指纹图谱建立及含量测定研究  

Study on UPLC fingerprint establishment and content determination of Hedyotidis chrysotricahae Herba

作　　者：胡剑虹 周海琴 曹杰楠 石海培 陈盛君[1] 李松[1] 海丰 张开雪 Hu Jianhong;Zhou Haiqin;Cao Jienan;Shi Haipei;Chen Shengjun;Li Song;Hai Feng;Zhang Kaixue(Jiangyin Tianjiang Pharmaceutical Co.,Ltd.,Jiangyin 214434,China)

机构地区：[1]江阴天江药业有限公司,江阴214434

出　　处：《国际中医中药杂志》2025年第2期229-236,共8页International Journal of Traditional Chinese Medicine

基　　金：江苏省科技成果转化专项资金项目(BA2021018)。

摘　　要：目的研究并建立金毛耳草UPLC指纹图谱和多指标成分含量测定方法,为金毛耳草药材质量控制提供参考。方法采用ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8µm)色谱柱;以乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,检测波长254 nm;流速0.30 ml/min;柱温35℃。建立了同时测定芦丁、山柰酚-3-O-芸香糖苷、水仙苷、新绿原酸、绿原酸、隐绿原酸含量及指纹图谱的方法,并结合指纹图谱相似度评价、聚类分析、主成分分析等对17批金毛耳草样品进行质量分析。结果建立了金毛耳草药材UPLC指纹图谱共有模式,标定了11个共有峰,指认了其中7个,主成分分析基本可将17批金毛耳草按照不同产地分为4类。不同产地金毛耳草药材各指标性成分含量略有差异,其中以贵州都匀产地的含量较高。结论本研究建立的UPLC指纹图谱和6个指标成分含量的测定方法可用于金毛耳草药材的质量评价和控制,为其标准完善提升提供理论依据。Objective To study and establish the UPLC fingerprint and multi-index content determination methods of Hedyotidis chrysotricahae Herba;To provide a reference for the quality control of Hedyotidis chrysotricahae Herba.Methods The chromatographic column was ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8µm).The mobile phase was acetonitrile-0.1%phosphoric acid solution with gradient elution;the detection wavelength was 254 nm;the flow rate was 0.30 ml/min and column temperature was 35℃.The method could determine content and fingerprint of rutin,Kaempferol-3-O-rutinoside,Narcissoside,Neochlorogenic aci,Chlorogenic Acid,Cryptochlorogenic acid and have quality analysis to 17 batches of Hedyotidis chrysotricahae Herba based on the variance of fingerprint,similarity evaluation,clustering analysis along with principal component analysis(PCA)at the same time.Results The common pattern of UPLC specific chromatogram of Hedyotidis chrysotricahae Herba was established.The 11 common peaks were marked out,among which 7 peaks were identified.17 batches Hedyotidis chrysotricahae Herba could be divided into 4 categories according to different origins.Quality content of six indicators of Hedyotidis chrysotricahae Herba was in slight difference between different origins,among which the content quality of Hedyotidis chrysotricahae Herba from Duyun in Guizhou Province was the highest.Conclusion The established UPLC fingerprint and content determination method of 6 indicators from the study can be used for the quality control of Hedyotidis chrysotricahae Herba,which can also provide a theoretical basis for the standard improvement of Hedyotidis chrysotricahae Herba.

关 键 词：含量测定(中药) 金毛耳草 指纹图谱 聚类分析 主成分分析 质量控制 

分 类 号：R28[医药卫生—中药学]