基于网络药理学探讨三仁汤治疗多囊卵巢综合征和2型糖尿病的“异病同治”作用机制  

Network Pharmacology-based Study on the Mchanism of Sanren Tang in the Treatment of PCOS and Type 2 Diabetes Mellitus

作　　者：王嘉萌 王博宇 丛慧芳[2] WANG Jiameng;WANG Boyu;CONG Huifang(Heilongjiang University of Chinese Medicine,Harbin 150040,China;The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150001,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001

出　　处：《西部中医药》2025年第3期48-58,共11页Western Journal of Traditional Chinese Medicine

摘　　要：目的:应用网络药理学方法探讨三仁汤治疗多囊卵巢综合征(polycystic ovary syndrome,PCOS)和2型糖尿病(type 2 diabetes mellitus,T2DM)的作用机制,为中药“异病同治”提供科学的理论依据。方法:利用中药数据库(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)、中医药综合数据库(traditional Chinese medicine integrated database,TCMID)、中医药整合药理学研究平台(integrative pharmacology-based research platform of traditional Chinese medicine,TCMIP)、中药成分靶点平台2.0(HIT 2.0)、传统中医分子机制生物信息学分析工具(bioinformatics analysis tool for molecular mechANism of traditional Chinese medicine,BATMAN-TCM)、草药-中医证候-西医症状-成分-靶标-疾病关联网络数据库(symmap integrates traditional Chinese medicine,SymMap)等平台获取和筛选三仁汤的活性成分,并判断其生物活性;得到有效活性成分对应的靶点基因,输入Uniprot数据库,将靶点标准化;利用人类基因组数据库(the Human Gene database,GeneCards)、药物靶标数据库(therapeutic target database,TTD)、比较毒物基因组学数据库(the comparative toxicogenomics database,CTD)和药物银行数据库(DrugBank)获取PCOS和T2DM疾病靶点,得到三仁汤治疗PCOS和T2DM的共同作用靶点;采用Cytoscape 3.7.1软件绘制三仁汤“异病同治”的“单味药-活性成分-共同靶点”网络图;利用STRING平台绘制蛋白相互作用网络;通过注释、可视化和综合发现数据库(the database for annotation,visualization and integrated discovery,DAVID)、R语言(R studio)等平台进行生物过程分析,获得Mode节点分析、基因本体论(gene ontology,GO)富集分析和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)富集分析结果。结果:共筛选得到三仁汤有效活性成分50个、PCOS疾病靶点23332个、T2DM疾病靶点28438个,三仁汤“异病同治”PCOS和T2DM的共同靶点214个;蛋�Objective:To provide science-based theoretical reference for"the same treatment for different diseases"in TCM by discussing the mechanism of Sanren Tang in the treatment of PCOS and type 2 diabetes mellitus(T2DM)based on network pharmacology.Methods:The platforms including TCMSP,TCMID,and TCMIP,HIT2.0,BATMAN-TCM,and SymMap,were applied to obtain and screen out the active ingredients of Sanren Tang,and to judge its biological activity;The target genes corresponding to the effective active components were obtained,entered into the Uniprot database to standardize the targets;GeneCards,TTD,CTD and DrugBank were utilized to obain the targets of PCOS and T2DM,and the common targets of the decoction in the treatment of PCOS and T2DM;Cytoscape 3.7.1 software was adopted to draw the network of"single herbactive ingredient-common targets"of"the same treatment for different diseases"in Sanren Tang;STRING was used to map protein interaction network;biological processes were conducted through DAVID,and R studio to perform Mode analysis,GO enrichment analysis and KEGG enrichment analysis.Results:The study has yielded 50 effective active ingredients of the decoction,23332 targets of PCOS,28438 targets of T2DM,and 214 targets of the treatment of PCOS and T2DM with Sanren Tang on the foundation of the same treatment for different diseases;39 key targets were identified for PPI;the results of GO and KEGG enrichment analysis showed that"the same treatment for different diseases"of Sanren Tang mainly involved inflammatory reaction,hypoxia reaction,apoptosis,HIF-1 signaling pathway,calcium ion channel and insulin resistance(IR);the results of MCODE analysis displayed that"the same treatment for different diseases"of Sanren Tang might be related to reactive oxygen species(ROS)response,apoptosis-related signaling pathway,IL-17 signaling pathway and fatty acid metabolism processes;the key targets of"the same treatment for different diseases"of Sanren Tang contained AKT1,TP53,CASP3,TNF and IL-6.Conclusion:Sanren Tang could develop the ef

关 键 词：多囊卵巢综合征 2型糖尿病 三仁汤 异病同治 

分 类 号：R259[医药卫生—中西医结合]