青钱柳叶中黄酮类化合物提纯、鉴定以及改善胰岛素抵抗活性  

Purification and Identification of Flavonoids from Cyclocarya paliurus Leaves and Ameliorative Effect on Insulin Resistance

作　　者：徐伟翔 张晴晴 席慧婷 刘忠伟 王远兴[1] XU Weixiang;ZHANG Qingqing;XI Huiting;LIU Zhongwei;WANG Yuanxing(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,江西南昌330047 [2]江西中医药大学药学院,江西南昌330004

出　　处：《食品科学》2025年第7期162-171,共10页Food Science

基　　金：国家自然科学基金地区科学基金项目(31960090;32160562);南昌大学食品科学与技术国家重点实验室自由探索项目(SKLF-ZZB-202129;SKLF-ZZB-202324)。

摘　　要：为开发用于治疗2型糖尿病的天然植物降血糖成分,本实验以药食同源植物青钱柳叶中的黄酮类化合物为研究对象,通过乙醇回流提取与聚酰胺柱纯化,得到棕黄色黄酮类化合物提取物。利用超高效液相色谱-四极杆飞行时间质谱鉴定出其中包含的17种黄酮类化合物,从中选择3种化合物标准品与青钱柳叶黄酮提取物(flavonoid extract of Cyclocarya paliurus leaves,FEC)分析其改善胰岛素抵抗活性。借助HepG2细胞建立胰岛素抵抗模型,通过优化得出最佳建模条件为葡萄糖质量浓度4.5 g/L、胰岛素浓度0.001μmol/L、地塞米松浓度3.5μmol/L,联合诱导HepG2细胞24 h,建立的胰岛素抵抗模型能稳定存在48 h,并且能够被临床降血糖药物二甲双胍治疗。基于胰岛素抵抗模型对黄酮类化合物进行研究发现,FEC、金丝桃苷、野黄芩苷在质量浓度为10^(-2)mg/mL时改善胰岛素抵抗活性最高,而山柰酚质量浓度为10^(-4)mg/mL时活性最高。进一步的研究表明,青钱柳叶黄酮类化合物可能是通过改善胰岛素抵抗模型的糖原代谢紊乱和提高氧化应激能力,从而起到改善胰岛素抵抗作用。In order to develop natural plant-derived hypoglycemic agents for the treatment of type 2 diabetes,an extract rich in flavonoids with a brownish-yellow color was obtained from the leaves of Cyclocarya paliurus,a medicinal and edible plant,by sequential refluxing ethanol extraction and polyamide column chromatography.In the extract,17 flavonoids were identified by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).Meanwhile,the flavonoid-rich extract and the standards of its three representative flavonoid components were evaluated for their ameliorative effect on insulin resistance.A HepG2 cell model of insulin resistance was established under optimized conditions(4.5 g/L glucose,0.001μmol/L insulin,3.5μmol/L dexamethasone,and 24 induction),which was stable for 48 h and could be treated with metformin,an oral hypoglycemic agent.The flavonoid-rich extract as well as hyperoside and scutellarin at a concentration of 10^(-2) mg/mL and kaempferol at 10^(-4) mg/mL were the most effective in ameliorating insulin resistance.Furthermore,we found that the mechanism for the ameliorative effect of flavonoids from Cyclocarya paliurus leaves on insulin resistance may be by alleviating glycogen metabolism disorders and increasing oxidative stress in the insulin resistance model.

关 键 词：青钱柳叶 黄酮类化合物 胰岛素抵抗 HEPG2细胞 

分 类 号：TS201.4[轻工技术与工程—食品科学]