健脾解毒汤对VEGF/VEGFR2通路影响及其对银屑病血管生成和炎症的作用研究  

Study on the Effects of Jianpi Jiedu Decoction on the VEGF/VEGFR2 Pathway and Its Role in Angiogenesis and Inflammation in Psoriasis

作　　者：姚文汇 左永杰 刘红霞[2] Yao Wenhui;Zuo Yongjie;Liu Hongxia(Xinjang Medical University,Xinjiang,Urumqi 830000,China.)

机构地区：[1]新疆医科大学,新疆乌鲁木齐830000 [2]新疆医科大学附属中医医院,新疆乌鲁木齐830000

出　　处：《中国中医急症》2025年第3期399-403,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金地区项目(81960871);新疆维吾尔自治区重点研发计划项目(2021B03001-2)。

摘　　要：目的从血管生成、炎症角度,探究健脾解毒汤对银屑病(脾虚湿盛证)大鼠的干预机制。方法选取6~8周龄的SPF级SD雄性大鼠,体质量180~200 g,共32只,采用随机数表法分为空白组、模型组、甲氨蝶呤组、健脾解毒汤组各8只。空白组以凡士林涂抹背部,其他组给予5%咪喹莫特(62.5 mg,每日2次)涂抹背部,并灌胃大黄颗粒剂(10 mL/kg,每日2次),共10 d。模型建立后,甲氨蝶呤组灌胃甲氨蝶呤片0.97 mg/kg,每日1次;健脾解毒汤组灌胃健脾解毒汤9.26 g/kg,每日1次;空白组、模型组予等量生理盐水。给药14 d后,比较4组PASI、组织病理情况,VEGF、VEGFR2、IL-6、TNF-αm RNA的表达,VEGF、VEGFR2、p-VEGFR2蛋白含量表达。结果空白对照组背部皮肤未见明显变化,健脾解毒汤组红斑、鳞屑等改善优于模型组。模型组皮肤结构异常,与模型组比较,健脾解毒汤组大鼠皮肤表皮增生、角化不全明显减轻,中性粒细胞数量减少以及真皮血管扩张减弱。模型组PASI评分高于空白对照组(P<0.01),甲氨蝶呤组、健脾解毒汤组评分低于模型组(P<0.01)。与模型组相比,健脾解毒汤组VEGF、VEGFR2、IL-6、TNF-αmRNA表达量降低(P<0.05);与模型组相比,甲氨蝶呤组VEGF蛋白表达量降低(P<0.05),健脾解毒汤组VEGF蛋白表达量降低(P<0.05);VEGFR2蛋白表达量4组之间无明显差异,而模型组p-VEGFR2蛋白表达量较对照组升高(P<0.05),健脾解毒汤组与模型组相比p-VEGFR2蛋白表达量降低(P<0.05)。结论健脾解毒汤在一定程度上不仅减轻银屑病(脾虚湿盛证)大鼠的病理改变,还与降低炎症因子,抑制大鼠皮损部位血管生成作用相关。Objective:To investigate the mechanism of Jianpi Jiedu Decoction in treating rats with psoriasis(syndrome of dampness excess due to spleen deficiency) from the perspectives of angiogenesis and inflammation.Methods:A total of 32 male SPF-grade SD rats aged 6~8 weeks and weighing 180~200 g were randomly divided into four groups(8 rats per group):blank control group,model group,methotrexate group,and Jianpi Jiedu Decoction group. The blank control group was treated with petroleum jelly applied to the back,while the other groups were treated with 5% imiquimod cream(62.5 mg,twice daily)applied to the back and rhubarb granules(10 mL/kg,twice daily)for 10 days. After the model was established,the methotrexate group was administered methotrexate tablets(0.97 mg/kg,once daily),and the Jianpi Jiedu Decoction group was given Jianpi Jiedu Decoction(9.26 g/kg,once daily)by gavage. The blank control group and the model group were given an equal amount of normal saline.After 14 days of treatment,the following were compared among the four groups:Psoriasis Area and Severity Index(PASI),histopathological changes,mRNA expression levels of VEGF,VEGFR2,IL-6,TNF-α,and protein expression levels of VEGF,VEGFR2,and p-VEGFR2. Results:No significant changes were observed in the skin of the blank control group. The Jianpi Jiedu Decoction group showed better improvement in erythema and scaling compared with the model group. Histopathological examination revealed abnormal skin structure in the model group.Compared with the model group,the Jianpi Jiedu Decoction group showed positive effects in reducing epidermal hy- perplasia,incomplete keratinization,neutrophil infiltration,and dermal vascular dilation. The model group had sig-nificantly higher scores than the blank control group(P < 0.01),while both the methotrexate group and the Jianpi Jiedu Decoction group had significantly lower scores than the model group(P < 0.01). Compared with the modelgroup,the Jianpi Jiedu Decoction group exhibited reduced m RNA expression levels of VEGF,VEGFR2,IL

关 键 词：银屑病 健脾解毒汤 血管生成 炎症 大鼠 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]