文冠果TCP基因家族鉴定及盐胁迫下表达分析  

Identification of TCP gene family in Xanthoceras sorbifolium and expression analysis under salt stress

作　　者：陈玉金 于涵 朱华利 姜涛 刘博 李伟 赵永军 鲁仪增 解孝满 CHEN Yu-jin;YU Han;ZHU Hua-li;JIANG Tao;LIU Bo;LI Wei;ZHAO Yong-jun;LU Yi-zeng;XIE Xiao-man(College of Landscape and Forestry,Qingdao Agricultural University/Shandong Key Laboratory for Germplasm Innovation of Saline-alkaline Tolerant Grasses and Trees,Qingdao,Shandong 266109,China;Jinan Lily Landscape Group Co.,Ltd.,Jinan,Shandong 250014,China;Key Laboratory of State Forestry and Grassland Administration on Conservation and Utilization of Warm Temperate Zone Forest and Grass Germplasm Resources/Shandong Center of Forest and Grass Germplasm Resources,Jinan,Shandong 250102,China)

机构地区：[1]青岛农业大学园林与林学院/山东省耐盐碱草木种质创新重点实验室,山东青岛266109 [2]济南百合园林集团有限公司,山东济南250014 [3]暖温带林草种质资源保存与利用国家林业和草原局重点实验室/山东省林草种质资源中心,山东济南250102

出　　处：《南方农业学报》2025年第2期441-451,共11页Journal of Southern Agriculture

基　　金：山东省重点研发计划(农业良种工程)项目(2020LZGC0904);山东省自然资源厅珍贵树种保育利用创新团队项目(鲁自然资字〔2023〕98号)。

摘　　要：【目的】对文冠果(Xanthoceras sorbifolium)TCP基因家族进行鉴定,分析其在盐胁迫下的表达情况,探究文冠果的盐胁迫响应机制,为文冠果耐盐种质的选育提供理论依据。【方法】采用生物信息学方法对文冠果TCP基因家族进行鉴定,并进行蛋白理化性质、系统发育进化和保守基序分析,以及基因结构、染色体定位和启动子顺式作用元件分析。采用实时荧光定量PCR检测盐胁迫下文冠果TCP基因(XsTCPs)在叶片和根部的表达情况。【结果】共鉴定出24个文冠果TCP基因家族成员(XsTCP1~XsTCP24),编码的氨基酸残基数量为185~576个,蛋白分子量为20576.21~63366.92 kD,理论等电点为4.62~10.25,均属于亲水性蛋白且定位于细胞核。基于系统发育进化分析结果,可将24个XsTCPs蛋白划分到3个亚家族,其中PCF亚家族包含14个成员、CIN亚家族包含6个成员、CYC/TB1亚家族包含4个成员。除XsTCP23和XsTCP24基因无法定位到染色体外,其余文冠果TCP基因家族成员以单个基因或基因簇的形式不均匀地分布在11条染色体上。在24个XsTCPs蛋白中共发现10个保守基序,其中Motif 1为所有蛋白共有。文冠果TCP基因家族成员含有光响应元件、激素反应元件、应激反应元件和植物生长发育作用元件,其中XsTCP4、XsTCP6、XsTCP7、XsTCP10、XsTCP12、XsTCP13、XsTCP21和XsTCP22基因含有干旱诱导的MYB结合元件;实时荧光定量PCR检测结果表明,盐胁迫下上述基因在文冠果叶片和根部的表达情况均发生明显变化,推测上述8个XsTCPs基因参与盐胁迫响应过程。【结论】XsTCPs蛋白可能通过调控涉及生长发育、新陈代谢和应激反应的多种靶基因参与生物学过程,推测含有干旱诱导的MYB结合元件的8个XsTCPs基因有助于提高文冠果对盐胁迫的抵御能力。【Objective】The TCP gene family of Xanthoceras sorbifolium was identified and its expression under salt stress was analyzed,its response mechanism to salt stress was studied,which provided theoretical basis for breeding of salt-tolerant germplasm of X.sorbifolium.【Method】The TCP gene family of X.sorbifolium was identified by bioinforma-tics method,and the protein physicochemical properties,phylogenetic evolution,conserved motifs,gene structure,chromosome localization and promoter cis-acting elements were analyzed.Real-time fluorescence quantitative PCR was used to detect the expression of TCP genes of X.sorbifolium(XsTCPs)in leaf and root under salt stress.【Result】A total of 24 members of the TCP gene family(XsTCP1-XsTCP24)of X.sorbifolium were identified,encoding 185-576 amino acids residues,protein molecular weight being 20576.21-63366.92 kD,theoretical isoelectric point being 4.62-10.25,all of which were hydrophilic proteins and located in the nucleus.Based on the phylogenetic analysis results,the 24 XsTCPs proteins could be divided into 3 subfamilies,among which the PCF subfamily contained 14 members,the CIN subfamily contained 6 members,and the CYC/TB1 subfamily contained 4 members.Except that XsTCP23 and XsTCP24 genes were unable to locate on the chromosome,the remaining TCP gene family members were unevenly distributed on 11 chromosomes in the form of single genes or gene clusters.A total of 10 conserved motifs were found in 24 XsTCPs proteins,among which Motif 1 was common to all proteins.The TCP gene family members of X.sorbifolium contained light response elements,hormone response elements,stress response elements and plant growth and development elements.XsTCP4,XsTCP6,XsTCP7,XsTCP10,XsTCP12,XsTCP13,XsTCP21 and XsTCP22 genes contained drought-induced MYB binding elements.The results of real-time fluorescence quantitative PCR showed that the expression of the above genes changed greatly in the leaves and roots of X.sorbifolium under salt stress,suggesting that the above 8 XsTCPs genes were inv

关 键 词：文冠果 TCP基因家族 盐胁迫 表达分析 

分 类 号：S662.9[农业科学—果树学]