基于16S rDNA测序的硅沉着病小鼠肺部和肠道菌群特征分析  

Analysis on characteristics of lung and intestinal flora in silicotic mice based on 16S rDNA sequencing

作　　者：杨思思 唐小翠 赵晓航 王娜[1] 姚武[1] YANG Sisi;TANG Xiaocui;ZHAO Xiaohang;WANG Na;YAO Wu(Department of Occupational Health,College of Public Health,Zhengzhou University,Zhengzhou 450001)

机构地区：[1]郑州大学公共卫生学院劳动卫生学教研室,郑州450001

出　　处：《郑州大学学报(医学版)》2025年第2期158-161,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金面上项目(81773404)。

摘　　要：目的:探讨硅沉着病小鼠肺部和肠道菌群特征。方法:将20只雄性C57BL/6N小鼠随机分为对照组和SiO_(2)组,每组10只,SiO_(2)组采用吸入式气管滴注SiO_(2)悬液法构建硅沉着病模型。于第56天处死小鼠,采用实时荧光定量PCR法检测肺组织中IL-6、IL-1β、TNF-α、α-SMA、COL1A1和TGF-βmRNA的表达,采用Western blot法检测肺组织中α-SMA蛋白的表达;收集肺泡灌洗液和粪便样本,采用16S rDNA测序方法检测肺部和肠道菌群的丰度和α多样性。结果:与对照组相比,SiO_(2)组小鼠肺组织中IL-6、IL-1β、TNF-α、COL1A1、TGF-β、α-SMA mRNA表达水平和α-SMA蛋白表达水平均升高(P<0.05)。两组小鼠肺部和肠道菌群存在共同的差异菌群且变化相反,α多样性指标比较差异无统计学意义(P>0.05)。SiO_(2)组小鼠疣微菌门、阿克曼菌属和阿克曼菌等在肠道减少而在肺部增加(P<0.05)。结论:硅沉着病小鼠肺部和肠道菌群发生变化并影响硅沉着病的发生发展。Aim:To investigate the characteristics of lung and intestinal flora in mice with silicosis.Methods:A total of 20 male C57BL/6N mice were randomly divided into control group and SiO_(2)group of 10 mice each.The SiO_(2)group was injected SiO_(2)suspension into the pharynx to establish silicosis model.On day 56,the mice were euthanized.The expression levels of IL-6,IL-1β,TNF-α,α-SMA,COL1A1 and TGF-βmRNA were detected by real-time fluorescence quantitative PCR.The expression level ofα-SMA protein was detected by Western blot.Bronchoalveolar lavage fluid and fecal samples were collected,and abundance andαdiversity of lung and intestinal flora were detected by 16S rDNA sequencing.Results:Compared with control group,the expression levels of IL-6,IL-1β,TNF-α,COL1A1,TGF-β,α-SMA mRNA andα-SMA protein in lung tissue of the SiO_(2)group all elevated(P<0.05).Lung and intestinal flora shared common differential flora and varied in opposite directions.There was no significant difference in the indexes ofαdiversity of the flora between the 2 groups(P>0.05).The mice in SiO_(2)group had a decrease in the intestine and an increase in the lung of Verrucomicrobia,Ackermannia and Akkermansia muciniphila(P<0.05).Conclusion:The lung and intestinal flora are altered in mice with silicosis,which affects the development of silicosis.

关 键 词：硅沉着病 肺纤维化 肺部菌群 肠道菌群 16S rDNA测序 小鼠 

分 类 号：R135.2[医药卫生—劳动卫生]