高良姜素调节Hippo/Yes相关蛋白信号通路对糖尿病骨质疏松大鼠骨代谢影响的研究  

Study on the effect of galangin on bone metabolism by regulating Hippo/Yes-related protein signaling pathway in rats with diabetic osteoporosis

作　　者：黄碧[1] 郝梦 欧翔 邵挥戈[1] HUANG Bi;HAO Meng;OU Xiang(Department of Endocrinology,Changsha Central Hospital,Changsha 410028,China)

机构地区：[1]长沙市中心医院内分泌科,410028

出　　处：《中国糖尿病杂志》2025年第2期136-141,共6页Chinese Journal of Diabetes

基　　金：湖南创新型省份建设专项任务项目(2023JJ30066)。

摘　　要：目的 探讨高良姜素(GAL)调节Hippo/Yes相关蛋白(YAP)信号通路对糖尿病骨质疏松(DOP)大鼠骨代谢的影响。方法 90只雌性SD大鼠随机分为正常对照(NC)组、模型(Mod)组、低剂量GAL(GAL-L)组、高剂量GAL(GAL-H)组和高剂量GAL+YAP抑制剂Verteporfin(GAL-H+Verteporfin)组,每组18只。双能X射线检测骨密度(BMD),三点弯曲实验评估股骨生物力学,HE染色观察股骨组织病理变化,ELISA检测血清骨钙素(OC)、碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRAP)水平,Western blot法检测股骨组织YAP表达。结果 与NC组比较,Mod组股骨BMD、最大载荷、弹性模量、OC、ALP、YAP表达降低(P<0.05),TRAP升高(P<0.05)。与Mod组比较,GAL-L、GAL-H组股骨BMD、最大载荷、弹性模量、OC、ALP、YAP表达升高(P<0.05),TRAP降低(P<0.05),且GAL-H组改善程度更优。与GAL-H组比较,GAL-H+Verteporfin组股骨BMD、最大载荷、弹性模量、OC、ALP、YAP表达降低(P<0.05),TRAP升高(P<0.05)。结论GAL可能通过调控Hippo/YAP信号通路,提高YAP表达,调节DOP大鼠骨代谢,抑制骨吸收。Objective To explore the effect of galangin(GAL)on bone metabolism by regulating Hippo/Yes associated protein(YAP)signaling pathway in rats with diabetic osteoporosis(DOP).Methods 90 female SD rats were randomly divided into normal control(NC)group,model(Mod)group,low-dose GAL(GAL-L)group,high-dose GAL(GAL-H)group,and high-dose GAL+YAP inhibitor Verteporfin(GAL-H+Verteporfin)group,with 18 rats in each group.Dual energy X-ray was used to detect bone mineral density(BMD).Three-point bending assay was used to evaluate the biomechanics of femur.HE staining was used to test the pathological changes in femoral tissue.ELISA was used to detect serum levels of osteocalcin(OC),alkaline phosphatase(ALP),and tartaric-resistant acid phosphatase(TRAP).Western blot was used to evaluate the expression of YAP protein in femoral tissue.Results Compared with NC group,the BMD,maximum load,elastic modulus,OC,ALP,and YAP protein expression of femur were reduced(P<0.05),and the TRAP was increased in Mod group(P<0.05).Compared with Mod group,BMD,maximum load,elastic modulus,OC,ALP and YAP protein expressions of femur were increased(P<0.05),while TRAP was decreased in GAL-L and GAL-H groups(P<0.05),and the improvement was better in GAL-H group.Compared with GAL-H group,femur BMD,maximum load,elastic modulus,OC,ALP and YAP protein expressions of femur were decreased(P<O.05),while TRAP was increased in GAL-H+Verteporfin group(P<0.05).Conclusions GAL may regulate the Hippo/YAP signaling pathway,increase the expression of YAP,regulate bone metabolism in DOP rats,and inhibit bone resorption.

关 键 词：高良姜素 Hippo/Yes相关蛋白信号通路 糖尿病骨质疏松 骨代谢 

分 类 号：R587.2[医药卫生—内分泌] R580[医药卫生—内科学]