蓝光通过脑-肾轴对大鼠肾结石形成的影响研究  

Effects of blue light through the brain-kidney axis of rats on the formation of kidney stones

作　　者：方道成 谢莉皊 全霖杰 张奎[1] 温晖 FANG Daocheng;XIE Liling;QUAN Linjie;ZHANG Kui;WEN Hui(Department of Urology,Songjiang Hospital Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai 201600,China)

机构地区：[1]上海交通大学医学院附属松江医院泌尿外科,上海201600

出　　处：《现代泌尿外科杂志》2025年第3期257-260,共4页Journal of Modern Urology

基　　金：上海市松江区科技攻关项目(No.2023SJKJGG84)。

摘　　要：目的分析蓝光通过脑-肾轴对大鼠肾结石形成的影响。方法将40只大鼠随机分为A、B、C、D 4组,每组10只。C、D组大鼠采用10 g/L乙二醇+20 g/L氯化铵+100 g/L葡萄糖酸钙(2 mL/只)进行灌胃,A、B组大鼠给予等体积量生理盐水灌胃。A、C组大鼠从灌胃后第2天开始每日进行2次蓝光照射(1 h/次)干预,B、D组采用日光灯照射(方法同上),持续4周。收集大鼠24 h尿液后处死并收集其血液及肾脏组织样本。检测大鼠血清抗利尿激素(ADH)、尿Ca^(2+)和尿草酸(Oxa)水平,ELISA法检测大鼠肾脏组织丙二醛(MDA)、超氧化物歧化酶(SOD)水平。Von Kossa染色观察肾组织病变以及肾脏中钙盐结晶情况。结果与A、B组相比,C、D组大鼠肾脏组织中钙盐结晶积分较高,血清ADH、尿Ca^(2+)、尿Oxa及肾组织中MDA水平较高,而肾组织SOD水平较低(P<0.05);与D组相比,C组肾脏组织中钙盐结晶积分较高,血清ADH、尿Ca^(2+)、尿Oxa及肾组织中MDA水平较高,而肾组织SOD水平较低(P<0.05)。结论蓝光可能通过脑-肾轴促进血清ADH分泌和肾脏组织氧化应激反应,进而促进大鼠肾结石的形成。Objective To analyze the effects of blue light on the formation of kidney stones.Methods A total of 40 rats were randomly divided into 4 groups:A,B,C,and D,with 10 rats in each group.Rats in groups C and D were administered with a mixture of 10 g/L ethylene glycol,20 g/L ammonium chloride,and 100 g/L calcium gluconate via gavage(2 mL per mouse),while rats in groups A and B received an equal volume of physiological saline via gavage.From the second day after gavage,rats in groups A and C were subjected to twice-daily blue light irradiation(one hour per session)as an intervention,while rats in groups B and D were subjected to fluorescent lamp irradiation using the same method.After 4 weeks of intervention,the 24-hour urine samples were collected,and the rats were then euthanized for the collection of blood and kidney tissue samples.Serum levels of antidiuretic hormone(ADH),urinary Ca^(2+),and urinary oxalate(Oxa)were measured.Levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in kidney tissues were detected using ELISA.Von Kossa staining was performed to observe pathological changes in kidney tissues and the presence of calcium salt crystals in the kidneys.Results Compared with groups A and B,groups C and D showed higher accumulation of calcium salt crystals in renal tissues,as well as elevated levels of ADH,urinary Ca^(2+),urinary Oxa,and MDA in renal tissues,additionally,the SOD level in renal tissues was lower(P<0.05).Compared with group D,group C exhibited higher accumulation of calcium salt crystals in renal tissues,along with increased levels of ADH,urinary Ca^(2+),urinary Oxa,and MDA in renal tissues;conversely,the SOD level in renal tissues was lower(P<0.05).Conclusion Blue light may increase the formation of kidney stones in rats by promoting the secretion of ADH in serum and oxidative stress in kidney tissues through the brain-kidney axis.

关 键 词：蓝光 脑-肾轴 大鼠 肾结石 抗利尿激素 丙二醛 超氧化物歧化酶 

分 类 号：R692.4[医药卫生—泌尿科学]