慢性阻塞性肺疾病患者外周血单核细胞中miRNA-146a表达与氧化应激水平及炎症反应的关系研究  

Study on the Relationship between miRNA-146a Expression in Peripheral Blood Mononuclear Cells and Oxidative Stress Levels and Inflammatory Response in Patients with Chronic Obstructive Pulmonary Disease

作　　者：田璐 阮峥 郭雨平[2] Tian Lu;Ruan Zheng;Guo Yuping(The Second Department of Internal Medicine,Daye Iron Mine Hospital of Huangshi,Wuhan Nanshan Healthcare Co.,Ltd.,Huangshi,Hubei,435006,China;Department of Respiratory Medicine,Huangshi Central Hospital,Huangshi,Hubei,435000,China)

机构地区：[1]武汉楠山康养有限责任公司黄石大冶铁矿医院内二科,湖北黄石435006 [2]黄石市中心医院呼吸内科,湖北黄石435000

出　　处：《哈尔滨医药》2025年第1期1-4,共4页Harbin Medical Journal

摘　　要：目的探讨慢性阻塞性肺疾病患者外周血单核细胞(PBMCs)中miRNA-146a表达与氧化应激水平及炎症反应的关系。方法收集52例COPD患者及52例健康者的外周血样本,采用实时荧光定量PCR技术检测PBMCs中miRNA-146a的表达,生化试剂盒检测氧化应激标志物的水平,以及ELISA法检测炎症反应指标的水平,比较指标的相关性。结果与健康对照组相比,COPD患者PBMCs中miRNA-146a的表达水平显著降低(P<0.05)。随着GOLD分级的升高,miRNA-146a的表达水平呈下降趋势。COPD患者血浆中MDA水平显著高于健康对照组(P<0.05),而SOD、GSH-Px水平则显著低于健康对照组(P<0.05)。COPD患者血浆中IL-6、TNF-α、IL-1β水平显著高于健康对照组(P<0.05)。COPD患者PBMCs中miRNA-146a的表达水平与MDA水平呈负相关(均P<0.05),与SOD、GSH-Px水平呈正相关(P<0.05)。同时,miRNA-146a的表达水平与IL-6、TNF-α、IL-1β水平也呈负相关(均P<0.05)。结论miRNA-146a的表达水平与COPD的严重程度密切相关,且与氧化应激水平及炎症反应指标存在显著的相关性。miRNA-146a可能参与了COPD的发病过程。Objective The primary objective of this study was to explore the intricate relationship between the expression of miRNA-146a in peripheral blood mononuclear cells(PBMCs)of patients afflicted with chronic obstructive pulmonary disease(COPD)and both the oxidative stress levels and the inflammatory response.Methods Peripheral blood samples were procured from 52 individuals diagnosed with COPD and 52 healthy counterparts.The expression of miRNA-146a in PBMCs was detected using real-time fluorescence quantitative PCR technology.Biochemical assay kits were employed to ascertain the levels of oxidative stress markers,and ELISA was utilized to measure the levels of inflammatory response markers.Subsequently,a comprehensive comparison of the correlations among these indicators was conducted.Results In contrast to the healthy control group,a significant reduction in the expression level of miRNA-146a in the PBMCs of COPD patients was observed(P<0.05).Notably,as the GOLD grading advanced,a downward trend in the expression level of miRNA-146a became evident.The levels of malondialdehyde(MDA)in the plasma of COPD patients were markedly higher than those in the healthy control group(P<0.05),while the levels of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were substantially lower than those in the healthy control group(P<0.05).Additionally,the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)in the plasma of COPD patients were significantly elevated compared to those in the healthy control group(P<0.05).Intriguingly,the expression level of miRNA-146a in the PBMCs of COPD patients exhibited a negative correlation with MDA levels(both P<0.05),and a positive correlation with SOD and GSH-Px levels(P<0.05).Concurrently,the expression level of miRNA-146a was inversely correlated with the levels of IL-6,TNF-α,and IL-1β(all P<0.05).Conclusion The expression level of miRNA-146a is intimately intertwined with the severity of COPD and demonstrates a significant correlation with oxid

关 键 词：慢性阻塞性肺疾病 miRNA-146a 外周血单核细胞 氧化应激 炎症反应 

分 类 号：R563.5[医药卫生—呼吸系统]