青杞1号外植体再生培养基的筛选  

Screening of Regeneration Medium for Explant of Qingqi No.1

作　　者：杨帅 郑贞贞 段国珍 李建领 樊光辉[2,3] YANG Shuai;Zheng Zhenzhen;DUAN Guozhen;LI Jianling;FAN Guanghui(College of Agriculture and Animal Husbandry,Qinghai University,Xining Qinghai 810016,China;Academy of Agricultural and Forestry Sciences,Qinghai University,Xining Qinghai 810016,China;Key Laboratory of Forest Genetics and Breeding in Qinghai Plateau,Xining Qinghai 810016,China)

机构地区：[1]青海大学农牧学院,青海西宁810016 [2]青海大学农林科学院,青海西宁810016 [3]青海省高原林木遗传育种重点实验室,青海西宁810016

出　　处：《青海农林科技》2025年第1期107-112,共6页Science and Technology of Qinghai Agriculture and Forestry

基　　金：青海省科技厅重大专项(2023-NK-A4)。

摘　　要：为了筛选适合青杞1号外植体再生培养基以及缩短组培周期,本试验以青杞1号的叶片、茎段为外植体,研究了不同处理培养基对愈伤组织诱导、不定芽的分化的影响。结果表明,在7种培养基中,叶片愈伤组织诱导和分化的最适培养基为1/2MS+0.5 mg·L^(-1)6-BA+0.3 mg·L^(-1) IBA,愈伤组织诱导率为100%,不定芽诱导率为64.75%;茎段愈伤组织诱导和分化的最适培养基为1/2 MS+1.0 mg·L^(-1)6-BA+1.0 mg·L^(-1) IBA,愈伤组织诱导率为91.04%,不定芽诱导率为54.94%;青杞1号叶片与茎段相比更适合作为外植体构建再生体系;通过试验获得最佳叶片诱导培养基1/2MS+0.25 mg·L^(-1)6-BA+0.15 mg·L^(-1) IBA,愈伤组织出愈率为100%,不定芽分化率为95.60%;将不定芽转接至MS培养基中进行生根诱导,生根率为100%。综上,本研究为建立青杞1号遗传转化体系提供技术支持。In order to screen a regenerating medium suitable for qingqi No.1 explants and to shorten the tissue culture period,The effects of different media on callus induction and adventitious bud differentiation were studied using the leaves and stems of qingqi No.1 as explants.The results showed that the optimal medium for callus induction and differentiation was 1/2MS+0.5 mg·L^(-1)6-BA+0.3 mg·L^(-1) IBA,with a callus induction rate of 100%and an adventitious bud induction rate of 64.75%.For stem segments,the optimal medium for callus induction and differentiation was 1/2MS+1.0 mg·L^(-1)6-BA+1.0 mg·L^(-1) IBA,resulting in a callus induction rate of 91.04%and an adventitious bud induction rate of 54.94%.Compared to stem segments,the leaves of qingqiNo.1 were more suitable as explants for constructing a regeneration system.Through orthogonal testing,the optimal leaf induction medium was found to be 1/2MS+0.25 mg·L^(-1)6-BA+0.15 mg·L^(-1) IBA,achieving a callus induction rate of 100%and an adventitious bud differentiation rate of 95.60%.Adventitious buds were then transferred to MS medium for rooting induction,with a callus itduction rate of 100%.In conclusion,this study provides technical support for establishing a genetic transformation system for qingqi No.1.

关 键 词：青杞1号 组织培养 植株再生 

分 类 号：S567.53[农业科学—中草药栽培]