BD FACSAria流式分选仪无菌制备流程的优化  

Optimization of the Aseptic Preparation Process for BD FACSAria Flow Sorter

作　　者：杨欢 郭英华[1,2] 张雪芳 罗佳 杜立颖 吕红霞[1,2] YANG Huan;GUO Yinghua;ZHANG Xuefang;LUO Jia;DU Liying;LYU Hongxia(School of Life Sciences,Peking University,Beijing 100871,China;National Center for Protein Sciences(Beijing)at Peking University,Beijing 100871,China)

机构地区：[1]北京大学生命科学学院,北京100871 [2]国家蛋白质研究中心(北京)北京大学分中心,北京100871

出　　处：《实验科学与技术》2025年第2期128-132,共5页Experiment Science and Technology

摘　　要：为优化BD FACSAria流式分选仪无菌制备和无菌维护的流程,以保持设备长期稳定的无菌分选状态,从无菌制备的成本、操作流程和系统稳定性等方面进行优化,探索了LB培养基监测管路无菌状态的方法,并对HCT116细胞无菌分选状态及长期培养情况进行观察。优化后的无菌制备方法简化了操作流程、节约了成本,能够长期保持仪器液管的无菌状态;HCT116结直肠癌细胞分选后在无双抗培养基中长期培养状态良好且未出现污染的情况。优化的BD FACSAria分选仪无菌制备方法及维护流程为仪器的运行和管理提供了简单可行的方案,为科研工作提供更加优质稳定的无菌分选技术服务。To optimize the process of aseptic preparation and maintenance for BD FACSAria cell sorter to maintain long-term stable sterile sorting state,the cost of aseptic preparation,operation process and system stability are optimized.The method of monitoring the aseptic status of the fluidics system with LB medium is explored,and the aseptic sorting status and long-term culture of HCT116 cells are observed.The optimized aseptic preparation methods simplify the operation process,save the cost,and can keep the aseptic state of the instrument liquid flow pipeline for a long time.HCT116 colorectal cancer cells are sorted and cultured in antibioticfree medium for a long time without contamination.The optimized aseptic preparation methods and maintenance procedure of BD FACSAria sorter provide a simple and feasible scheme for the operation and management of the instrument,and provide high-quality and stable aseptic sorting services for scientific research.

关 键 词：流式分选仪 无菌制备优化 无菌维护 无菌分选 

分 类 号：TH74[机械工程—光学工程]