超大孔海绵体生物炭灭活大肠杆菌(Escherichia coli)研究  

Study on Inactivation of Escherichia coli by Ultra-macroporous Sponge-like Biochar

作　　者：龙玉娇 靳红梅[1,2,3] 张晶 LONG Yu-jiao;JIN Hong-mei;ZHANG Jing(Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Agro-environment in Downstream of Yangtze Plain,Ministry of Agriculture and Rural Affairs,Nanjing 210014,China;Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing 210095,China;Jiangsu Agricultural Waste Treatment and Recycle Engineering Research Center,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院农业资源与环境研究所/农业农村部长江下游平原农业环境重点实验室,江苏南京210014 [2]江苏省有机固体废弃物资源化协同创新中心,江苏南京210095 [3]江苏省农业废弃物资源化工程技术研究中心,江苏南京210014

出　　处：《生态与农村环境学报》2025年第3期430-436,共7页Journal of Ecology and Rural Environment

基　　金：江苏省农业科学院资环所青年联合创新项目[ZCX(2022)13]。

摘　　要：发展高效杀菌技术对农产品产地环境微生物污染控制具有重要意义。传统杀菌技术存在稳定性和持续性不高、易产生消毒副产物、成本较高等难题。该研究开发了一种基于超大孔海绵体生物炭的新型杀菌方法,并研究了其消毒特性与机理。结果表明,体系条件对消毒效果有显著影响,超大孔海绵体柚子皮生物炭投加量和硫酸盐浓度提高,细菌灭活效率随之提高。当柚子皮生物炭投加量为4 g·L^(-1)、硫酸盐浓度为50 mmol·L^(-1)时即可实现细菌的快速灭活(1 h灭活99.81%,2 h灭活99.97%)。通过对比研究柚子皮生物炭体系与柚子皮体系、椰壳生物炭体系的杀菌效果及孔径分布,发现此方法中柚子皮生物炭的超大孔结构与细菌尺寸相适配,能较好地捕集细菌或将捕集的细菌接触式灭活。柚子皮生物炭体系与无硫酸盐体系、氯盐体系及滤液体系杀菌效果的对比研究结果表明,柚子皮生物炭和硫酸盐溶液混合后可能会溶出具有杀菌作用的芳香性酚类或酮类化合物等化学物质,进一步提高杀菌效果。研究可为土壤等产地环境生物污染高效消毒技术创新提供参考方法。Developing efficient disinfection technology is of great significance to control environment biological pollution of agricultural products.Traditional disinfection technologies have problems of low stability,low sustainability,producing disinfection by-products and high cost.Herein,a new disinfection method based on ultra-macroporous sponge-like biochar was developed,and the performance and mechanism were investigated.Results show that operation conditions have significant influence on disinfection effects.With the increase of ultra-macroporous sponge-like pomelo peel biochar dosage and sulfate concentration,the inactivation efficiency of bacteria increased.Rapid inactivation could be achieved(99.81%inactivation in 1 hour and 99.97%inactivation in 2 hours)when dosage of pomelo peel biochar was 4 g·L^(-1)and sulfate concentration was 50 mmol·L^(-1).Bactericidal effects and pore size distribution of pomelo peel biochar system,pomelo peel system and coconut shell biochar system were compared.It was found that the ultra-macroporous structure in pomelo peel biochar is irreplaceable,which is compatible with bacteria size and could trap bacteria well or kill the captured bacteria by contact.Moreover,comparisons between pomelo peel biochar system,sulfate-free system,chlorine salt system and filtrate system suggest that mixture of pomelo peel biochar and sulfate solution would generate chemicals with bactericidal effect such as aromatic phenols or ketones,which further improved the bactericidal efficiency.This study can provide methodological support for technology innovation of efficient disinfection for soil biological pollution.

关 键 词：生物污染 超大孔海绵体 生物炭 柚子皮 消毒 

分 类 号：X53[环境科学与工程—环境工程] X52