次级视觉皮质N-甲基-D-天冬氨酸受体对甲基苯丙胺诱发情境关联学习记忆的影响  

Effect of N-methyl-D-aspartic acid receptor in secondary visual cortex on methamphetamine-associated contextual learning and memory

作　　者：孙楚 曹丹旎 宋睿 李锦 SUN Chu;CAO Danni;SONG Rui;LI Jin(Nanjing University of Chinese Medicine,Nanjing 210023,China;Beijing Key Laboratory of Neuropsychopharmacology,State Key Laboratory of National Security Specially Needed Medicines,Academy of Military Medical Sciences,Beijing 100850,China)

机构地区：[1]南京中医药大学,江苏南京210023 [2]军事医学研究院国家安全特需药品全国重点实验室,神经精神药理学北京市重点实验室,北京100850

出　　处：《中国药理学与毒理学杂志》2025年第3期161-168,共8页Chinese Journal of Pharmacology and Toxicology

基　　金：北京市自然科学基金(7232106);北京市科协青年人才托举工程(BYESS2023230)。

摘　　要：目的 研究次级视觉皮质(V2)脑区N-甲基-D-天冬氨酸(NMDA)受体对甲基苯丙胺情境关联学习记忆的影响。方法 以雄性C57BL/6J小鼠为研究对象,采用小鼠条件性位置偏爱(CPP)实验,分别观察形成期伴随V2脑区微注射、表达期测试前单次V2脑区微注射、小剂量甲基苯丙胺(0.5 mg·kg^(-1),ip)或环境线索点燃期测试前单次V2脑区微注射NMDA受体选择性拮抗剂D-AP5(双侧各0.5μg)后小鼠CPP得分,评价NMDA受体对甲基苯丙胺诱发CPP效应形成、表达和唤起的影响。结果与溶剂对照组相比,小鼠CPP形成期伴随甲基苯丙胺训练V2脑区微注射D-AP5(双侧各0.5μg)对CPP得分无显著抑制作用,表达期测试前单次V2脑区微注射D-AP5(双侧各0.5μg)对CPP得分无明显抑制作用,小剂量甲基苯丙胺(0.5 mg·kg^(-1),ip)点燃期测试前单次V2脑区微注射D-AP5(双侧各0.5μg)对CPP得分无明显抑制作用,环境线索点燃期测试前单次V2脑区微注射D-AP5(双侧各0.5μg)对CPP得分无明显抑制作用。结论V2脑区NMDA受体不参与甲基苯丙胺情境关联学习记忆的形成、表达和唤起。OBJECTIVE To investigate the effect of N-methyl-D-aspartic acid(NMDA)receptor in the secondary visual cortex(V2)on methamphetamine-associated contextual learning and memory.METHODS With male C57BL/6J mice as subjects and using the mouse conditioned place preference(CPP)experiment,the scores of CPP were observed after microinjection of NMDA receptor selective antagonist D-AP5(0.5μg per side)into the bilateral V2 during the formation phase,single microinjec-tion of D-AP5(0.5μg per side)into the bilateral V2 prior to the expression test,and methamphetamine(0.5 mg∙kg^(-1),ip)-induced reactivation test and methamphetamine-associated contextual-induced reacti-vation test,to evaluate the effect of NMDA receptors on the formation,expression and reinstatement of methamphetamine-induced CPP.RESULTS Compared with the control group,microinjection of D-AP5(0.5μg per side)into the bilateral V2 during the formation phase did not have significant inhibitory effect on CPP scores,nor did single microinjection of D-AP5(0.5μg per side)into the bilateral V2 prior to the expression test,single microinjection of D-AP5(0.5μg per side)into the bilateral V2 prior to metham-phetamine(0.5 mg∙kg^(-1),ip)-induced reactivation test or single microinjection of D-AP5(0.5μg per side)into the bilateral V2 prior to methamphetamine-associated contextual-induced reactivation test.CONCLU-SION The NMDA receptor in the V2 is not involved in the formation,expression and reactivation of meth-amphetamine-associated contextual learning and memory.

关 键 词：次级视觉皮质 条件性位置偏爱 甲基苯丙胺 N-甲基-D-天冬氨酸受体 

分 类 号：R964[医药卫生—药理学]