长链非编码RNA GAS5、NLR与D-二聚体联合检测对肺结核的诊断价值  

Diagnostic value of the combined detection of long non-coding RNA GAS5,NLR,and D-dimer in pulmonary tuberculosis

作　　者：刘海梅 魏雅静 朱慧明 王守卫 马佳慧 李峰 LIU Haimei;WEI Yajing;ZHU Huiming;WANG Shouwei;MA Jiahui;LI Feng(Department of Clinical Laboratory,Nantong Sixth People's Hospital,Jiangsu 226011)

机构地区：[1]南通市第六人民医院检验科,江苏226011

出　　处：《南通大学学报(医学版)》2025年第1期43-46,共4页Journal of Nantong University(Medical sciences)

基　　金：南通市卫健委科研项目(MS2024080,QN2024048)。

摘　　要：目的:评价长链非编码RNA生长抑制特异性转录本5(growth arrest-specific 5,GAS5)、中性粒细胞与淋巴细胞比值(neutrophil-to-lymphocyte ratio,NLR)和D-二聚体(D-dimer,DD)联合检测在肺结核中的诊断效能。方法:选取2024年1—10月收治的肺结核住院患者50例纳入观察组,选取同期健康体检者52人纳入对照组。对两组对象采用实时荧光定量PCR评估GAS5表达,血常规检测并计算NLR,同时检测DD水平。绘制ROC曲线评价联合检测GAS5、NLR和DD对肺结核的诊断效能。结果:观察组患者GAS5水平显著低于对照组(0.216 vs 0.904,P<0.001),NLR和DD水平均高于对照组(4.564 vs 1.688、0.795 mg/L vs 0.300 mg/L,均P<0.001)。3个指标联合检测的ROC曲线AUC为0.937,敏感度为92.00%,特异度为82.70%。结论:GAS5、NLR和DD联合检测对结核病的诊断具有较高的敏感度,联合诊断效能卓越,为肺结核早期诊断及后续相关治疗提供依据。Objective:To evaluate the diagnostic efficacy of the combined detection of long non-coding RNA growth arrest-specific 5(GAS5),neutrophil-to-lymphocyte ratio(NLR)and D-dimer(DD)in pulmonary tuberculosis.Methods:A total of 50 inpatients with pulmonary tuberculosis admitted from January to October 2024 were included in the observation group,and 52 patients were included in the control group.Real-time quantitative PCR was used to evaluate the expression of GAS5 in the two groups,and the NLR was detected and calculated by routine blood tests,and the level of DD was detected at the same time.The diagnostic efficacy of combined detection of GAS5,NLR and DD in pulmonary tuberculosis was evaluated.Results:The level of GAS5 in the observation group was significantly lower than that in the control group(0.216 vs 0.904,P<0.001),and the levels of NLR and DD were higher than those in the control group(4.564 vs 1.688 and 0.795 mg/L vs 0.300 mg/L,both P<0.001).The AUC of the ROC curve of the three indexes was 0.937,the sensitivity was 92.00%,and the specificity was 82.70%.Conclusion:The combined detection of GAS5,NLR and DD has high sensitivity and excellent diagnostic efficiency in the diagnosis of tuberculosis,which provides a basis for the early diagnosis of pulmonary tuberculosis and subsequent related treatment.

关 键 词：肺结核 长链非编码RNA生长抑制特异性转录本5 中性粒细胞与淋巴细胞比值 D-二聚体 联合诊断 

分 类 号：R521[医药卫生—内科学]