嗜线虫致病杆菌YZ011对鳞翅目昆虫杀虫活性及比较基因组学分析  

Insecticidal activity and comparative genomics analysis of Xenorhabdus nematophila YZ011 against lepidopteran insects

作　　者：林曼曼 刘琴[1] 韩光杰[1] 黄立鑫 夏杨 李传明[1] 张楠[2] 陆玉荣[1] 徐彬[1] 徐健[1,2] LIN Manman;LIU Qin;HAN Guangjie;HUANG Lixin;XIA Yang;LI Chuanming;ZHANG Nan;LU Yurong;XU Bin;XU Jian(Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province/National Agricultural Experimental Station for Agricultural Microbiology in Yangzhou,Yangzhou 225007,China;College of Plant Protection,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]江苏里下河地区农业科学研究所/国家农业微生物扬州观测实验站,江苏扬州225007 [2]扬州大学植物保护学院,江苏扬州225009

出　　处：《江苏农业学报》2025年第2期258-267,共10页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2024YFD1400900);江苏省农业科技自主创新基金项目[CX(22)1009];江苏省国际合作项目(BZ2020039);扬州市科技计划项目(YZ2023244);农业基础性长期性科技工作项目(NAES069AM04)。

摘　　要：昆虫病原线虫共生菌是一类重要的生物防治资源。为明确本单位分离自小卷蛾斯氏线虫共生菌YZ011对鳞翅目害虫的杀虫活性及机制,本研究在测定菌株YZ011对小菜蛾和草地贪夜蛾血腔注射毒性的基础上,进一步对其进行全基因组测序和比较基因组学分析。结果表明,血腔注射100 nL 1.0×10^(6)CFU/mL菌株YZ01124 h后,小菜蛾幼虫和草地贪夜蛾幼虫的死亡率分别为75.0%和56.7%,均显著高于血腔注射磷酸盐缓冲液(PBS)对照。全基因组测序结果显示菌株YZ011为嗜线虫致病杆菌,其基因组含有19个次级代谢产物基因簇;与其同源性最高的嗜线虫致病杆菌菌株YL001和菌株SⅡ相比,菌株YZ011含有29个特异基因簇。菌株YZ011基因组中共注释到586个毒力因子相关基因。本研究结果从基因组层面解析了嗜线虫致病杆菌YZ011的分子特征,对菌株YZ011的综合利用具有重要意义。Entomopathogenic nematode symbionts are important biological control resources.In order to clarify the insecticidal toxicity and mechanism of the symbiotic bacteria YZ011 isolated from Steinernema carpocapsae on lepidopteran pests,the whole genome sequencing and comparative genomics analysis of strain YZ011 were further carried out on the basis of measuring the hemocoel injection toxicity of strain YZ011 to Plutella xylostella and Spodoptera frugiperda.The results showed that the mortality rates of Plutella xylostella larvae and Spodoptera frugiperda larvae were 75.0%and 56.7%,respectively,after hemocoel injection of 100 nL 1.0×10^(6)CFU/mL strain YZ011 for 24 h,which were significantly higher than those of phosphate buffered saline(PBS)control.The whole genome sequencing results showed that YZ011 was a nematophagous pathogenic bacterium,and its genome contained 19 secondary metabolite gene clusters.Compared with the most homologous strains YL001 and SⅡ,YZ011 contained 29 specific gene clusters,and 586 virulence factor-related genes were annotated.The results of this study analyzed the molecular characteristics of Xenorhabdus nematophila YZ011 at the genomic level,which was of great significance for the comprehensive utilization of strain YZ011.

关 键 词：嗜线虫致病杆菌 杀虫活性 基因组 次级代谢产物 毒力因子 

分 类 号：S476[农业科学—农业昆虫与害虫防治]